Protein Info for B158DRAFT_1954 in Kangiella aquimarina DSM 16071

Annotation: protease FtsH subunit HflK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 transmembrane" amino acids 53 to 71 (19 residues), see Phobius details PF12221: HflK_N" amino acids 1 to 44 (44 residues), 50.5 bits, see alignment 1.5e-17 TIGR01933: HflK protein" amino acids 75 to 335 (261 residues), 277.7 bits, see alignment E=4.6e-87 PF01145: Band_7" amino acids 76 to 248 (173 residues), 96.4 bits, see alignment E=2.1e-31

Best Hits

Swiss-Prot: 40% identical to HFLK_VIBCH: Protein HflK (hflK) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 90% identity to kko:Kkor_0520)

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (353 amino acids)

>B158DRAFT_1954 protease FtsH subunit HflK (Kangiella aquimarina DSM 16071)
MAWNEPGNNNDQDPWGKKRPNNNNDLDKLLKQASEKFGGIFGGGKGNGKGSSGGNASFII
GLLVLVAIYLFKSAYTVDEKQNALVLTLGKHTRTDTAGLHFAFPPIQQVYLIDVESIKDI
EVEGLMLTKDDNVATVKVKVQYRVNDPLDYKFNVVDPVETLKHATEAALRQVIGHTRLQD
ARTDKKEEVRKNVENELKSILKPYNAGIEIFRLNLIGNVDVPPSVKPAFDDAIKAEEDQR
AYLEQGQAYRSKQIPLAEGEAQKLIQQANAYRARVIEKAAGEVARFEKLLPEYIAAPVVT
RQRLYIETIESVLSKSSKVMLDVEGGNNMTYIPLDSILKRNKTSNSDSNQQKQ