Protein Info for B158DRAFT_1949 in Kangiella aquimarina DSM 16071

Annotation: N-acetylmuramoyl-L-alanine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF11741: AMIN
  • amino acids 17 to 117 (101 residues), 62.6 bits, E=4.9e-21
• PF01520: Amidase_3
  • amino acids 140 to 359 (220 residues), 160.9 bits, E=4.7e-51
• PF01476: LysM
  • amino acids 382 to 424 (43 residues), 50.1 bits, 3.2e-17

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 91% identity to kko:Kkor_0515)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (9/14 steps found)
• peptidoglycan recycling II (4/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Protein Sequence (427 amino acids)

>B158DRAFT_1949 N-acetylmuramoyl-L-alanine amidase (Kangiella aquimarina DSM 16071)
MLTLGISIAQASVIQSMRFWQSPESTRVVLDLSSPVTHEISILENPHRIVVDIPGANVNV
DLNRLEIQSDLVKRVRKSSPPRDGVLRLVLDLNKAAKPKSFSLKPYQEYGDRLVIDLFDE
SREEVQPPVAAQGSDRDIVVAVDAGHGGEDPGAIGGRGTKEKDIVLALSKELVKELNKVE
GIKAFLTRTGDYYLPHRKRTDLARLQRADLFVSVHADGFKSPRAKGASVWVLNLHGAKSE
VARWMQMQEEKSELLGGVDSSVVLSNYDNSVKSVLLDLQMENSITESTKVANIVHGAMSK
VVPKMHKKHVEENSLLVLKNPDIPSILVELGFITNPEEEALMKTSSYRQKLARGVGDGIV
DYFKQHAPDGTLFAARYRQNIYHVQRGDSLIKVARRFNVSVRDLKSANQLSTNVVKIGQK
LVIPTAE