Protein Info for B158DRAFT_1869 in Kangiella aquimarina DSM 16071

Annotation: DNA-directed RNA polymerase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1355 TIGR02013: DNA-directed RNA polymerase, beta subunit" amino acids 8 to 1351 (1344 residues), 1906.9 bits, see alignment E=0 PF04563: RNA_pol_Rpb2_1" amino acids 27 to 500 (474 residues), 106.5 bits, see alignment E=4.1e-34 PF04561: RNA_pol_Rpb2_2" amino acids 151 to 222 (72 residues), 25.8 bits, see alignment 2.3e-09 amino acids 358 to 455 (98 residues), 57.7 bits, see alignment 3.9e-19 PF04565: RNA_pol_Rpb2_3" amino acids 514 to 581 (68 residues), 103.6 bits, see alignment 1.5e-33 PF10385: RNA_pol_Rpb2_45" amino acids 592 to 657 (66 residues), 87.5 bits, see alignment 1.4e-28 PF00562: RNA_pol_Rpb2_6" amino acids 719 to 1276 (558 residues), 462.1 bits, see alignment E=4.7e-142 PF04560: RNA_pol_Rpb2_7" amino acids 1278 to 1352 (75 residues), 97.9 bits, see alignment 1.2e-31

Best Hits

KEGG orthology group: K03043, DNA-directed RNA polymerase subunit beta [EC: 2.7.7.6] (inferred from 64% identity to aav:Aave_4531)

Predicted SEED Role

"DNA-directed RNA polymerase beta subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1355 amino acids)

>B158DRAFT_1869 DNA-directed RNA polymerase, beta subunit (Kangiella aquimarina DSM 16071)
MAYSYTEKKRIRKDFGRRSNILDVPYLLAIQKDSYREFLEPRGDEDSGLDSAFRSIFPIE
SYSGNAALEYVGYSLGEPVFNVKECQIRGITYAAPLRVKIRLVIYDKDSKTKKVKDIREQ
EVFMGELPLMTENGTFVINGTERVIVSQLHRSPGVFFDSDRGKTHSSGKLLYQARVIPYR
GSWLDFEFDPKDMLYVRIDRRRKLPASIILRSLGYNTQDMLDMFFERNNFYITKKGEVQL
ELIPSRLRGETATFEIKAKGKVVVEEGRRITPRHIAQLEKAGVKKLDVPTEYIYGRALAT
DIIDKESGEIIAEANAEITEEILEKMIEAGVTEVPTLYTNDLDHGPYMSDTLRIDSTRTP
LEAKVEIYRMMRPGEPPTQDAAEALFENLFFNLERYDLSRVGRMKFNRRVGRDEIEGEGT
LSNDDIIDVIKTLIDIRNGNGTVDDIDHLGNRRIRCVGEMAENQFRVGLVRVERAVKERL
SQAESENLMPQDLINAKPVSAAVKEFFGSSQLSQFMDQNNPLSEITHKRRVSALGPGGLT
RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLACYARTNQYGFLETPYRRVADGKP
TAEVDYLSAIDEGKFVIAQANANLDKNGAFSDDLVPSRFQGEFMLATPEQVQYMDVSPQQ
IVSVAASMIPFLEHDDANRALMGSNMQRQAVPTLRAEKPLVGTGMERTVAVDSGVVVAAK
RGGVVDQVDASRVVIRVNEDEVGDNDSGVDIYNFTKYTRSNQNTCINQRPIVKQGDIVAR
GDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILISEKVVQEDRFTSIHIQELTCI
SRDTKLGPEEITADIPNVGESALSKLDESGIVYIGAEVKPGDILVGKVTPKGETQLTPEE
KLLRAIFGEKASDVKDTSLRVGTSTHGTVIDVQVFTRDGVEKDARAKEIENAEVARAKKD
LNDEFRILAESIYQRARNLVMGCVGEKGPNKFKKGSKITADYLDSLEPAQYLEIQLRVEE
KQQALESLANYLAEQRKEYDKKFEVQKLKITQGDDLAPGVQKIVKVYLAVKRRIQPGDKM
AGRHGNKGVISTIVPIEDMPYDENGEPVDIVLNPLGVPSRMNIGQILETHLGWAAKGVGI
KIGELLDTGKAPKTVRTFLNKVYNHRDEKPVDLSEMTDDEVMAMAENLRAGVPMATPVFD
GATEEDIHAMYELAELPKTGQSWLYDGRTGDRFERPVTVGYMYMLKLNHLIDDKMHARST
GSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTRMYKNI
VDGDHRMEPGMPESFNVLTKEIRSLAINIELEHED