Protein Info for B158DRAFT_1662 in Kangiella aquimarina DSM 16071

Annotation: heavy metal sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details PF21085: CusS" amino acids 11 to 166 (156 residues), 157.7 bits, see alignment E=6.7e-50 TIGR01386: heavy metal sensor kinase" amino acids 13 to 466 (454 residues), 448.1 bits, see alignment E=2e-138 PF00672: HAMP" amino acids 194 to 243 (50 residues), 31.5 bits, see alignment 4.6e-11 PF00512: HisKA" amino acids 248 to 313 (66 residues), 48.1 bits, see alignment E=2.5e-16 PF02518: HATPase_c" amino acids 358 to 468 (111 residues), 93.7 bits, see alignment E=2.5e-30

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 60% identity to kko:Kkor_1291)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (478 amino acids)

>B158DRAFT_1662 heavy metal sensor kinase (Kangiella aquimarina DSM 16071)
MTSMKNSFVKRQISLTTRVMTFVALSIGVSLLGIGYLVNNAVKHHFAEQDAEELRVMTNA
VDRTLRQADDITLDIEETLSKAVSGHHGVYFQVWNDDKQLLYGPSTNDYESPLYTYNPVS
RIKAGNLYQWQAEGKTYRGVITQTQIGSFKYTIVTAIDMDAHIQFLKSFHRSLWLIMILA
GTVTLLAAWFGVHQGHAPLRGLSATMREIQADRLHVRLKPDDVPRELLGLVTSFNDMIGR
LEESFERLSHFSADIAHELRTPLTNIITQTQVILGKSRSMVEYRELHYSNLEELYRLSKM
VNDTLWLAKSEHGLLKPVREPLDINQEVCDLFDFFEALAEEKQIQLKLDGQMPLLKVDRV
MFQRALSNLLSNALRHSPVGARVIVYLSISNKGEPTISVRNSGPGIPDEHKLRIFDRFYR
VDPSRQRQSEGAGLGLAIVKSIVEAHGGRIDVVSSNGETAFTIKFPLTAVTRGDDLNI