Protein Info for B158DRAFT_1485 in Kangiella aquimarina DSM 16071

Annotation: folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 30 to 421 (392 residues), 319.4 bits, E=2.3e-99
• PF08245: Mur_ligase_M
  • amino acids 52 to 198 (147 residues), 29.5 bits, E=7.2e-11
• PF02875: Mur_ligase_C
  • amino acids 290 to 343 (54 residues), 25.6 bits, 1.3e-09

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 70% identity to kko:Kkor_1483)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) / Folylpolyglutamate synthase (EC 6.3.2.17)" in subsystem Folate Biosynthesis (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (41/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (438 amino acids)

>B158DRAFT_1485 folylpolyglutamate synthase/dihydrofolate synthase (Kangiella aquimarina DSM 16071)
MSASPDFETLDEWIAWLEQAHPVHQIELGLARIQSVANKLGLDKLSAPVITVAGTNGKGT
VVAAVESLAIEHGLSVASYTSPHLLKFNERIKFDGQPVSDQMLVEAFKYIAKYQGDVPLT
YFEFTTLVAFWLFKSQPLELIVLEVGLGGRMDAVNIIDPDVAVITSIGLDHVAWLGDTLE
KVAHEKAGIMRARRPAIIADPQTTELLLPEAEIRNANSCLAQKDYHYTETGQCWSFETKV
KGQNLSFNGLANNDLLVTNLAGALEAFVLLYSTKISEKAVANAFHKLQFPGRFQYLSKEP
AIIVDVAHNPDSAKVLNSKLQQLKGRGVDKVSAICGMLKDKDIASCLSQCTEVDNWYCID
LPGPRGAKGEELLAKLPEFARTDAKSYHFLVDAFDEYWQHQQQNEALVVFGSFVTVSLML
ESWLELQNKLATRKQNEA