Protein Info for B158DRAFT_1445 in Kangiella aquimarina DSM 16071

Annotation: Phenylalanine 4-hydroxylase (EC 1.14.16.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 TIGR01267: phenylalanine-4-hydroxylase" amino acids 13 to 256 (244 residues), 360.7 bits, see alignment E=1.6e-112 PF00351: Biopterin_H" amino acids 17 to 236 (220 residues), 172.1 bits, see alignment E=8.1e-55

Best Hits

Swiss-Prot: 64% identical to PH4H_PSEAE: Phenylalanine-4-hydroxylase (phhA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00500, phenylalanine-4-hydroxylase [EC: 1.14.16.1] (inferred from 97% identity to kko:Kkor_1520)

Predicted SEED Role

"Phenylalanine-4-hydroxylase (EC 1.14.16.1)" in subsystem Aromatic amino acid degradation or Pterin biosynthesis (EC 1.14.16.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.16.1

Use Curated BLAST to search for 1.14.16.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (267 amino acids)

>B158DRAFT_1445 Phenylalanine 4-hydroxylase (EC 1.14.16.1) (Kangiella aquimarina DSM 16071)
MGTGTKYKARPMDEDGFIHYTDEEHQVWHELITRQEKLIKGRACPEYFEGLDKINLPKDR
IPQLEEVSSVLRKETGWEVAWVPALINFDKFFALLADKKFPCATFIRTREEMDYLQEPDV
FHEIFGHCAMLTNPYFAEFTSAYGKLGYAASKEDRVFLARLYWFTVEFGLINTAEGQRIY
GGGILSSIGETPHSLENPDVIRKPFDPIDILRTPYRIDIMQPQYFVIDDFKQLFDLAKSD
VMSMVKEAKRLGLHKPLYPPKEEKMAS