Protein Info for B158DRAFT_1419 in Kangiella aquimarina DSM 16071

Annotation: citrate synthase (EC 2.3.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01798: citrate (Si)-synthase
  • amino acids 14 to 423 (410 residues), 769.8 bits, E=2.9e-236
• PF00285: Citrate_synt
  • amino acids 46 to 412 (367 residues), 483.7 bits, E=1.7e-149

Best Hits

Swiss-Prot: 66% identical to CISY_COXBU: Citrate synthase (gltA) from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

KEGG orthology group: K01647, citrate synthase [EC: 2.3.3.1] (inferred from 99% identity to kko:Kkor_1546)

MetaCyc: 66% identical to citrate synthase (Escherichia coli K-12 substr. MG1655)
Citrate (Si)-synthase. [EC: 2.3.3.1, 2.3.3.16, 2.3.3.3]

Predicted SEED Role

"Citrate synthase (si) (EC 2.3.3.1)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 2.3.3.1)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle III (animals) (8/10 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• mixed acid fermentation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• ethene biosynthesis V (engineered) (14/25 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.1 or 2.3.3.16 or 2.3.3.3

Protein Sequence (429 amino acids)

>B158DRAFT_1419 citrate synthase (EC 2.3.3.1) (Kangiella aquimarina DSM 16071)
MSQQTAKLSLPNGEELDLTVLSPTLGKDVIDVRALGKAGYFTYDPGYVSTGACESKITYI
DGEKGQLLYRGYPIEQLAEQSNFEEVAYLLLNGELPSKEEYENFVAKINNHTMVHQQMMN
FFNGFRRDAHPMAIMVSVVGALSAFYHDSLDINNPQHRDISALRLIAKVPTIAAMCYRYS
VGHPFVYPDNDMSLAENFLNMMFSMPTNDWKPDPVLVKAMDRIFILHADHEQNASTSTVR
LAGSSGANPFAAIASGIACLWGPAHGGANEACLNMLREIGDVSRIPEFIERAKDKNDPFR
LMGFGHRVYKNFDPRAKVMRESAHEVLDLLGKHDDPIFKVALELEKIALEDEYFIEKKLY
PNVDFYSGVILDAMGIPTNMFTVIFALARTVGWVSQWHEMIGEDDRRIGRPRQLYTGATE
RDYVDINKR