Protein Info for B158DRAFT_1345 in Kangiella aquimarina DSM 16071

Annotation: aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02333: 2-methylisocitrate dehydratase, Fe/S-dependent
  • amino acids 2 to 860 (859 residues), 1744.3 bits, E=0
• TIGR01341: aconitate hydratase 1
  • amino acids 17 to 857 (841 residues), 955.5 bits, E=2.1e-291
• PF00330: Aconitase
  • amino acids 65 to 533 (469 residues), 458.3 bits, E=3.5e-141
• PF00694: Aconitase_C
  • amino acids 657 to 789 (133 residues), 97.5 bits, E=8.3e-32

Best Hits

Swiss-Prot: 82% identical to ACND_SHEON: 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (acnD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 83% identity to amc:MADE_03372)

MetaCyc: 82% identical to 2-methylcitrate dehydratase (Shewanella oneidensis)
RXN-8979 [EC: 4.2.1.117]

Predicted SEED Role

"2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.2.1.79)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• TCA cycle III (animals) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• reductive TCA cycle I (6/11 steps found)
• mixed acid fermentation (9/16 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (11/19 steps found)
• ethene biosynthesis V (engineered) (14/25 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3, 4.2.1.79

Use Curated BLAST to search for 4.2.1.117 or 4.2.1.3 or 4.2.1.79

Protein Sequence (862 amino acids)

>B158DRAFT_1345 aconitate hydratase 1/2-methylisocitrate dehydratase, Fe/S-dependent (Kangiella aquimarina DSM 16071)
MNTNYRKKLEGTNLDYFDTREAVEAIQPGAYDKLPYTSKVLAENLVRRCDPESLTDSLKQ
IIERKRDLDFPWFPARVVCHDILGQTAFVDLAGLRDAIAEKGGDPSKVNPVVPTQLIVDH
SLAVEHAGFEKDAFEKNRAIEERRNEDRFHFINWCKTAFENVNVVPPGNGIMHQINLERM
SPVIQSRDGVAFPDTLVGTDSHTPMVDALGVIAVGVGGLEAESVMLGRASYMRLPDIIGV
ELKGKPQPGITSTDIVLAITEFLRNERVVSSYLEFFGEGASHLTLGDRATISNMTPEFGA
TAAMFYIDQQTIDYLKLTGRDDDNVKLVETYAKQTGLWADSLTGAEYERVLTFDLSSVGR
NIAGPSNPHARVSTSDLASKGIAKHIETEEGKMPDGACIIAAITSCTNTSNPRNMIAAGL
IARNANKLGLTRKPWVKTSLAPGSKAVTLYLEESKLLPELENLGFGVVGYACTSCNGMSG
ALDPKIKQEIEERDLYSTAVLSGNRNFDGRIHPHADQAFLASPPLVVAYAIAGTIRFDIE
KDVLGTDKDGNPVTLKDIWPSDEEVDAIIKEHVKPEQFRAVYEPMFNVKVDTGEKSNPLY
DWRPMSTYIRRPPYWEGALAGERTMKGMRPLAVLGDNITTDHLSPSNAIQKSSAAGAYLD
KMGLPEEDYNSYATHRGDHLTAQRATFANPKLLNEMVRNEDGSVKQGSLARLEPEGKEMR
MWETIETYMERKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEVIVAEGFERIHRTNLIGM
GVLPVEFVNGDTRKTYAIDGTETFDVEGEISPGANLMVIMHRKDGERVEIPVKCRLDTAE
EVSIYSAGGVLQRFAQDFLAAS