Protein Info for B158DRAFT_1342 in Kangiella aquimarina DSM 16071

Annotation: fumarase, class I, homodimeric (EC 4.2.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05681: Fumerase
  • amino acids 12 to 284 (273 residues), 329.3 bits, E=1.8e-102
• TIGR00722: hydrolyase, tartrate alpha subunit/fumarate domain protein, Fe-S type
  • amino acids 13 to 282 (270 residues), 221.8 bits, E=9.9e-70
• PF05683: Fumerase_C
  • amino acids 288 to 490 (203 residues), 297.1 bits, E=5.3e-93
• TIGR00723: hydrolyase, tartrate beta subunit/fumarate domain protein, Fe-S type
  • amino acids 325 to 489 (165 residues), 163.3 bits, E=5.2e-52

Best Hits

KEGG orthology group: K01676, fumarate hydratase, class I [EC: 4.2.1.2] (inferred from 98% identity to kko:Kkor_0997)

Predicted SEED Role

"Fumarate hydratase class I, aerobic (EC 4.2.1.2)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VIII (Chlamydia) (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle III (animals) (8/10 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle I (6/11 steps found)
• incomplete reductive TCA cycle (3/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• mixed acid fermentation (9/16 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (11/19 steps found)
• pyruvate fermentation to propanoate I (2/7 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (9/17 steps found)
• L-glutamate degradation VIII (to propanoate) (2/11 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (3/13 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (16/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (501 amino acids)

>B158DRAFT_1342 fumarase, class I, homodimeric (EC 4.2.1.2) (Kangiella aquimarina DSM 16071)
MATIKAEDFIASVADAFQYISYYHPKDFIQALTAAYEKEESPAAKDAMKQILVNSRMSAM
GHRPVCQDTGIACAFVKVGMDARFEGGMTIQEMVDEGVRRAYLNPDNPLRASVLMDPAGK
RLNSKDNTPAVVHVEMVKGDKVEVTCAAKGGGSENKSKFVMLNPSDSIVDWVVETVPKMG
AGWCPPGMLGIGVGGTAEKAMVLAKESLMDPIDMQELLKRGPSNRVEELRIEIYEAVNKL
GIGAQGLGGVTTVLDVKIKDWPTHAASKPVAMIPNCAATRHIHFTLDGTGPAMLTPPSIE
DWPDMEYEAAADAKRVDMNNITPEEVASWKPGQVLLLNGKILTGRDAAHKKIKDLLDSGE
GLPEGLDFTNRFIYYVGPVDAVRDEAVGPAGPTTATRMDKFTEMMLDKTGLLGMIGKAER
GDEAIEAIKKHKSVYLMAVGGAAYLVSKAIKDSKVVAFPELGMEAVYEFDVKDMPVTVAV
DSEGESVHKTGPAVWKVKLAS