Protein Info for B158DRAFT_1297 in Kangiella aquimarina DSM 16071

Annotation: [LSU ribosomal protein L3P]-glutamine N5-methyltransferase (EC 2.1.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 14 to 296 (283 residues), 397.3 bits, see alignment E=3.8e-123 TIGR00536: methyltransferase, HemK family" amino acids 16 to 300 (285 residues), 262.9 bits, see alignment E=3e-82 PF03602: Cons_hypoth95" amino acids 126 to 216 (91 residues), 31 bits, see alignment E=7.1e-11 PF13847: Methyltransf_31" amino acids 134 to 211 (78 residues), 42.6 bits, see alignment E=1.9e-14 PF05175: MTS" amino acids 134 to 216 (83 residues), 58.9 bits, see alignment E=1.8e-19 PF06325: PrmA" amino acids 134 to 208 (75 residues), 26 bits, see alignment E=2.2e-09 PF13649: Methyltransf_25" amino acids 137 to 220 (84 residues), 39.5 bits, see alignment E=2.7e-13 PF08241: Methyltransf_11" amino acids 138 to 208 (71 residues), 24.7 bits, see alignment E=1.1e-08

Best Hits

Swiss-Prot: 56% identical to PRMB_SHEON: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 95% identity to kko:Kkor_0955)

MetaCyc: 54% identical to ribosomal protein L3 N5-glutamine methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-1241 [EC: 2.1.1.298]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.298 or 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (304 amino acids)

>B158DRAFT_1297 [LSU ribosomal protein L3P]-glutamine N5-methyltransferase (EC 2.1.1.-) (Kangiella aquimarina DSM 16071)
MTLSPIYQEAEQSLETVADFIRFAATQFHQSDLYFGHGTDNAWDEAVAIVLQMLDLPQDY
PQGMLTAKILADEKKHLSHAIFERVEHRKPLAYITNKAYFCGLEFYIDERVLVPRSPIAE
LIENDFYPWLEADNPRILDLCCGSGCIGLAIAAYIEDAEVVLSDISEDAITVADINNERL
GLYPKAQTAVSDLFESLADEQFDLIVSNPPYVDSEDLSDMPAEYQHEPEIGLGSGEDGLD
ITRRILEQAAEHLTEQGCLIVEVGNSWPALEEAFPDVPFTWLEFEKGGDGVFLLTRQQLV
EHFS