Protein Info for B158DRAFT_1261 in Kangiella aquimarina DSM 16071

Annotation: phenylalanyl-tRNA synthetase beta subunit (EC 6.1.1.20)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 791 (791 residues), 900.4 bits, see alignment E=5.8e-275 PF01588: tRNA_bind" amino acids 45 to 147 (103 residues), 83 bits, see alignment E=3.3e-27 PF03483: B3_4" amino acids 214 to 387 (174 residues), 245.8 bits, see alignment E=5.2e-77 PF03484: B5" amino acids 409 to 473 (65 residues), 68.2 bits, see alignment E=1.5e-22 PF17759: tRNA_synthFbeta" amino acids 476 to 683 (208 residues), 214.6 bits, see alignment E=3e-67 PF03147: FDX-ACB" amino acids 698 to 791 (94 residues), 105.2 bits, see alignment E=5e-34

Best Hits

Swiss-Prot: 57% identical to SYFB_PSEHT: Phenylalanine--tRNA ligase beta subunit (pheT) from Pseudoalteromonas haloplanktis (strain TAC 125)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 96% identity to kko:Kkor_1710)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (792 amino acids)

>B158DRAFT_1261 phenylalanyl-tRNA synthetase beta subunit (EC 6.1.1.20) (Kangiella aquimarina DSM 16071)
MKFSEKWLREWVNPEISTQQLVEKLTMAGLEVDGFEYLGNKFSGVVVGEILEAEQHPDAD
KLRVCKVSVGSASDEDLQIICGAPNARAGIKVAVAMIGAVLPGDFKIKKAKLRGVASHGM
LCSSTELEIGEDGDGILELDSQAPLGQDLKDYFDLNDHVIEVDLTPNRGDCLGIRGIARE
VGVLTQTAVTEHKPQALTPEISDVIPVKLSAPQACPRYLGRIIKGINPEAKTPDWMVKYI
ERSGVRSIDPVVDVTNYVLLELGHPMHAFDLKQIEGGIDVRMAKQGEKLTLLDGQEVELT
DDTLLIADHKKPLAIAGVMGGEHSGINTETDNIFLESAYFDPIAIAGRARQYGLHTDASH
RYERGVDFELQRTAMERATELLLEIVGGQPGDIIEAVDHDAMPTKRHLRLRRQRIERVVG
FPFADDQVEDILTRLGLKLTANADGWDVLVPSFRFDIDNEESLIEELVRVYGYNKLPSRK
PQAEMAMFDQPENRVWKNDMRDVLVKRGYQEVVTYSFVEPEFQLKLHPGIEPLPLINPIS
SEMAVMRTNLLPGLVNTAKFNINRQQNRIRIFELGLRFINSGDTLQQIPTIAGLVCGLRQ
KESWNTDGEKVDFYDLKGDVEALLQATSKTYSFETLHMDILHPGQAAEIIVDGHAVGYIG
KIHPQVQQEIDLDMDAYVFQMDLAPLSERKLPEFTPLSKFPSIRRDLALVVEDSVKAGEL
LDFIVKNGGNLLTDAHIFDIYKGEHLEPGKKSIALAMTLRHPDKTLEDAEINAVVDKVVS
GLAEQYGAALRN