Protein Info for B158DRAFT_1164 in Kangiella aquimarina DSM 16071

Annotation: valyl-tRNA synthetase (EC 6.1.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 923 transmembrane" amino acids 472 to 493 (22 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 904 (904 residues), 1234.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 598 (585 residues), 510.6 bits, see alignment E=7.7e-157 PF09334: tRNA-synt_1g" amino acids 38 to 88 (51 residues), 26.6 bits, see alignment 6.4e-10 amino acids 329 to 421 (93 residues), 21.6 bits, see alignment E=2.2e-08 PF13603: tRNA-synt_1_2" amino acids 238 to 299 (62 residues), 35.9 bits, see alignment 1.4e-12 PF08264: Anticodon_1" amino acids 641 to 796 (156 residues), 152.7 bits, see alignment E=1.7e-48 PF10458: Val_tRNA-synt_C" amino acids 856 to 920 (65 residues), 61.5 bits, see alignment 2e-20

Best Hits

Swiss-Prot: 61% identical to SYV_ECOL6: Valine--tRNA ligase (valS) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 66% identity to aeh:Mlg_0555)

MetaCyc: 61% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (923 amino acids)

>B158DRAFT_1164 valyl-tRNA synthetase (EC 6.1.1.9) (Kangiella aquimarina DSM 16071)
MEKAYDPKSIEQNLYKTWEDNDYFSPKGGDDSYCIMIPPPNVTGSLHMGHAFQHTIMDAL
TRYHRMKGDNTLWQPGSDHAGIATQMVVERLLDKEGKSRHDLGRDAFIDKVWEWKEHSGG
TITRQMRRLGDSCDWSREAFTMDDDLSDAVLEVFVRLYEEGLIYRGQRLVNWDPKLLTAV
SDLEVESHEENGHLWHLKYPLSDGSGHLVVATTRPETMLGDTAVAVHPEDERYQHLIGKT
IKLPLTDREIPIIADDYVEKDFGSGCVKITPAHDFNDYEMGQRHNLEMINVLTPTAHISD
TAPEAYRGLDRYEARKKIVEDFEQLGLLEKIEPHKLKVPRGDRSGVVIEPYLTDQWYVKI
APLAEPALKAVKDGDIKFVPENWSNTYYHWMENIQDWCISRQLWWGHQIPAWYDNDGNVY
VGRSEEDVRQKHNLGDIKLRRDEDVLDTWFSSALWPFATLGWPEETPELETFLPTSVLVT
GFDIIFFWVARMIMMGLKFTGKVPFKEIYITGLIRDEHGQKMSKSKGNVLDPIDIIDGID
LDSLVEKRTTGMMNPKDAEKIAKRTRKEFPEGIAPYGTDAMRFTFCAMASTSRDINFDLN
RVEGYRNFCNKIWNAARYVLMNTEEQDTGINGGEMTLSVADHWIKSELQKTITEFEKAVA
NYRFDLASNALYEFTWNTYCDWYLELSKPILYGDEYSEAEKRGTRHTLVSVLESLMRLLH
PFMPFITEEIWQRVAPLTGIDTKNHASIMLQPFPVSQAGLINDQAEQDIEWLKTIIVGVR
NIRGEMNIAPGKPLNVLFRNGSKQDKSRLDSYRTFLSKLAKLDSIEWLEAGQEAPMSATA
LAGKMEILIPMAGLIDVEQEMARLNKEKEKLEKEQQRISGKLSNKNFTDKAPEAVVNKEK
EKLEEVESTLGKVAEQIKQLEAL