Protein Info for B158DRAFT_1153 in Kangiella aquimarina DSM 16071

Annotation: histidyl-tRNA synthetase (EC 6.1.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 TIGR00442: histidine--tRNA ligase" amino acids 6 to 407 (402 residues), 524.5 bits, see alignment E=9.6e-162 PF13393: tRNA-synt_His" amino acids 10 to 312 (303 residues), 156.8 bits, see alignment E=1.3e-49 PF00587: tRNA-synt_2b" amino acids 68 to 318 (251 residues), 89 bits, see alignment E=6e-29 PF03129: HGTP_anticodon" amino acids 328 to 413 (86 residues), 45.4 bits, see alignment E=1.1e-15

Best Hits

Swiss-Prot: 68% identical to SYH_ALTMD: Histidine--tRNA ligase (hisS) from Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 92% identity to kko:Kkor_1837)

MetaCyc: 65% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (423 amino acids)

>B158DRAFT_1153 histidyl-tRNA synthetase (EC 6.1.1.21) (Kangiella aquimarina DSM 16071)
MAEKIQAVRGMNDILPDSTPHWQKLESVLRQLMASYGYSEIRMPVVEKTHLFKRSIGEVT
DIVEKEMYTFEDRNGESLTLRPEGTASCVRAGIEHGLLYNQEQRLWYMGPMFRHERPQKG
RYRQFYQLGVETFGVGTPDVDAELILMTARLWKKIGIHDSVRLEINSLGSNEARANYRDK
LVEFLLPIKDQLDEDSQRRLQTNPLRILDSKNPEVQKLVKDAPKLSEHLDDESKQHFERL
CQILDTNNIPYTINTNLVRGLDYYNRTVFEWITDKLGAQGTICAGGRYDGLTEQLGGHAT
PASGFAMGLERIVALIEEMETDEIPLADVYMVALGDQAELTATQLAEQLRDQWPDIKVRM
NCGAGNFKKQFKRADKSGAKIALVIGEQEVKDGTVGVKYLREHKEQLVISQRDVCQLLDQ
YLV