Protein Info for B158DRAFT_1028 in Kangiella aquimarina DSM 16071

Annotation: methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)/5,10-methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 121 (116 residues), 140 bits, E=4.2e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 124 to 280 (157 residues), 237.5 bits, E=4.4e-75

Best Hits

Swiss-Prot: 68% identical to FOLD_HAHCH: Bifunctional protein FolD (folD) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 97% identity to kko:Kkor_1959)

MetaCyc: 61% identical to bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase (Escherichia coli K-12 substr. MG1655)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• folate transformations II (plants) (9/11 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formaldehyde oxidation VII (THF pathway) (3/4 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• folate transformations I (7/13 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (3/15 steps found)
• purine nucleobases degradation II (anaerobic) (8/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (16/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (284 amino acids)

>B158DRAFT_1028 methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)/5,10-methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) (Kangiella aquimarina DSM 16071)
MSAQILDGKAISEKVKLQLAEKVKARLDKGLRAPGLAVILVGVDPASQIYVNKKVEACEK
VGFISRKIVLDATTTQEELLKHIDQLNADPEIDGILVQLPLPEHLDSNVILERIKADKDV
DGFHPYNMGRLAQKMPTMRPCTPYGVMVMLKETGIDLMGKDAVVIGVSNIVGRPMGLELL
MERCTVTFCHSKTKDLPKKVSEADIVVAGVGIPEMVKGDWIKPGAIVIDVGINRLESGKL
VGDVEFDVAKEKASWITPVPGGVGPMTVAMLMSNTLFACEHLHD