Protein Info for B158DRAFT_0979 in Kangiella aquimarina DSM 16071

Annotation: DNA topoisomerase IV, A subunit, proteobacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 6 to 741 (736 residues), 1159 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 30 to 466 (437 residues), 491.9 bits, see alignment E=1.7e-151 PF03989: DNA_gyraseA_C" amino acids 593 to 636 (44 residues), 19.8 bits, see alignment 4.4e-08 amino acids 646 to 691 (46 residues), 17.1 bits, see alignment 3.1e-07

Best Hits

Swiss-Prot: 71% identical to PARC_PSEAE: DNA topoisomerase 4 subunit A (parC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 95% identity to kko:Kkor_2007)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (746 amino acids)

>B158DRAFT_0979 DNA topoisomerase IV, A subunit, proteobacterial (Kangiella aquimarina DSM 16071)
MNYEGIEKKSLAEFTEQAYLNYSMYVIMDRALPHIGDGLKPVQRRIVYAMSELGLKATAK
YKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPKSFAAMR
YTESRLAAYSEVLLSELGQGTVNWIPNFDGTMDEPEVLPARIPNLLLNGTTGIAVGMATD
IPPHNLSEVGKACIHLLENPKSDVEDLLEFIQGPDFPTDAEIITPRSEILEVYEKGRGSI
RMRARFEKEEGDIVITALPHQVSGAKVLEQIAAQMQAKKLPMVSDLRDESDHENPTRLVI
EPRSNRVDVEELMRHLFATTDLERTYRVNMNMIGIDGRPQVKNLRVMLKEWLEFRHETVR
RRLQHRLDKVMDRLHILDGLLIAYLNIDEVIHIIRTEDKPKLKLMERFDLSDRQAEAILE
LKLRHLAKLEEMKIKGEQAELEDEADWLQLTLGSKQRMKTLIKKEIEEVVEQFGDERRSP
IVERKEAKALSETELLPTEPVTVILSEKGWVRCAKGHDVDAVGMNYRSGDKYLDSARGKS
NQYAVFLDSTGRTFSLLANTLPSARGLGEPVTGRVNPIAGAEFMATLMGEDDDKFLFATD
AGYGFVAKFSDLVTKTRNGKAFLSLPSGSKVVKPSSVQDPEKQFCVAVSNEGRMLVFPLA
DLPELAKGKGNKIISIPTARLKLREEFMVAVTAINEDDSLVIHSGKRHLTLKAKDLEHYR
GERGRRGHKLPRGFQRVDRIEVETKD