Protein Info for B158DRAFT_0972 in Kangiella aquimarina DSM 16071

Annotation: 3-deoxy-D-manno-octulosonic-acid transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04413: Glycos_transf_N
  • amino acids 35 to 210 (176 residues), 211.3 bits, E=4.1e-67

Best Hits

KEGG orthology group: K02527, 3-deoxy-D-manno-octulosonic-acid transferase [EC: 2.-.-.-] (inferred from 77% identity to kko:Kkor_2014)

Predicted SEED Role

"Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also]" (EC 2.4.99.12, EC 2.4.99.13)

MetaCyc Pathways

• superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
• Kdo transfer to lipid IV_A (Brucella) (2/2 steps found)
• Kdo transfer to lipid IV_A (E. coli) (2/2 steps found)
• Kdo transfer to lipid IV_A (H. pylori) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. gingivalis) (2/2 steps found)
• Kdo transfer to lipid IV_A (P. putida) (2/2 steps found)
• Kdo transfer to lipid IV_A (generic) (2/2 steps found)
• Kdo transfer to lipid IV_A (Haemophilus) (1/2 steps found)
• Kdo transfer to lipid IV_A (Vibrio cholerae serogroup O1 El Tor) (1/2 steps found)
• Kdo transfer to lipid IV_A (Chlamydia) (2/5 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (14/25 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis
• Puromycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.4.99.12 or 2.4.99.13

Protein Sequence (415 amino acids)

>B158DRAFT_0972 3-deoxy-D-manno-octulosonic-acid transferase (Kangiella aquimarina DSM 16071)
MLRYLYTLLYILAAPLLCVRWVYKSFKPPQYREPLRERFGIVSKESSESIWFHAVSMGES
NAAIAIIKSLLDKFPDLNIIVTTTTPTGARQIHNGLGDKVKHHYSPCDLPGTIKRFIRRA
RPKLLVIMETELWPNWLHHLKSSQIPVILANGRLSERSAQKYEKICSLSKEMMDAFVKVL
AVNNQDAERFQRIGLTSDKAIVTGNIKFDINIPQFSDHEYYPVWKGRTVWIAASTHKGED
ELVLKAHQLICEQVPEACLILVPRHPERFESVARLISDSGLSMARRSQPDSWDEGAQVLL
GDTMGEMMKAFNISDITWVGGSFAEIGGHNPIEPAVLAKPVLTGPYIFNFNEIFGELIAA
GGAQMVQNESELADKIVELMKTPELAHGMGQKALQVIERNRGALDKTVSVIEQYL