Protein Info for B158DRAFT_0941 in Kangiella aquimarina DSM 16071

Annotation: S-formylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 6 to 277 (272 residues), 407.3 bits, E=1.7e-126
• PF00756: Esterase
  • amino acids 23 to 271 (249 residues), 240.2 bits, E=4.3e-75
• PF00326: Peptidase_S9
  • amino acids 132 to 276 (145 residues), 33.9 bits, E=3.5e-12

Best Hits

Swiss-Prot: 59% identical to SFGH2_ECOK1: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli O1:K1 / APEC

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 87% identity to kko:Kkor_2047)

MetaCyc: 58% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (3/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (3/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Protein Sequence (278 amino acids)

>B158DRAFT_0941 S-formylglutathione hydrolase (Kangiella aquimarina DSM 16071)
MSINEISANRSFGGWHKQFTHTSSVLNCEMRFAVYLPPQMEQGKNVPVLYWLSGLTCTDE
NFMQKAGAHRMAAELGMMIVAPDTSPRGDDVADDEGYDLGKGAGFYVNATQEPWAKHYQM
YDYVTRELPQIIEGNFSVNNKKAILGHSMGGHGALMIALRNPDMFVSASAFSPIVNPMNC
PWGKKAFTAYLGSDEQEWQQYDSCFLLEQAEKQIPMLVSQGDADQFLTEQLKPETLQAVA
RKKNYPLILEIHQGYDHSYYFIASFIEQHLKFHAEHLA