Protein Info for B158DRAFT_0936 in Kangiella aquimarina DSM 16071

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 574 (573 residues), 441.6 bits, E=2.5e-136
• PF13522: GATase_6
  • amino acids 33 to 166 (134 residues), 128.6 bits, E=3.2e-41
• PF13537: GATase_7
  • amino acids 50 to 171 (122 residues), 132.2 bits, E=2.1e-42
• PF00733: Asn_synthase
  • amino acids 252 to 625 (374 residues), 299.9 bits, E=7.8e-93

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 98% identity to kko:Kkor_2050)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-asparagine biosynthesis (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (670 amino acids)

>B158DRAFT_0936 asparagine synthase (glutamine-hydrolyzing) (Kangiella aquimarina DSM 16071)
MCGIAGVIHRDKDRTIDAQTLVNMAAIQYHRGPDNFGYYNPEGVGVGMSHARLTIIDLDE
ERGRQPLVSDDQRYMMVHNGEFYDFQRIRADMTAQGARYQTKSDSEIVLQMYPHYGIEKT
VENLRGEFAFSIYDREEETMYLVRDRFGIKPLYWTETVHGVVFGSELKVLFAHPEVKREI
DSEGLYHQLIQVMIPGSTAFKGVNQVPPGHWIKIQRRNGKLEISEHKYWDIDFPQAEAHL
NVKDEQEYIDGVRENLLEAVQHRLNADVPVGAYLSGGIDSCSILGLSASVRQDPVKAFTI
GFDDADYDETPIAREMAEMTGAEQIILPLSAEHLYDHFERTIWHTERTIYNTLGVAKLLM
SQKVREQGYKVVLTGEGSDELFAGYPAFRNDMFLYGLDDLPENLRKEMQSTLAESNKLFK
GAMLPREAFRSEAIDAKVGFTPTCIQNWMGCDGFARQLMSKDHQANISDYDPGTAMAEQF
DENQLEGRHPLDKAQYVWIKTMLEGQILTWGGDRVDMANSMEARPPFLDHHLAAYAVNIP
PQMRIKGPVEKYVLKEAMKGLLPETLYKRQKFAFMAPPAHTDKKKWNALMELMEEFASEE
KVAAAGLIDYGALQDMIKKNDDPTVPREDKVQMDAILNHVLGVQLLHHHFIENDVPKMAA
AKAHELGWVA