Protein Info for B158DRAFT_0840 in Kangiella aquimarina DSM 16071

Annotation: L-proline dehydrogenase (EC 1.5.99.8)/delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1052 PF18327: PRODH" amino acids 22 to 65 (44 residues), 24.8 bits, see alignment (E = 4.2e-09) PF14850: Pro_dh-DNA_bdg" amino acids 74 to 187 (114 residues), 156 bits, see alignment E=9.6e-50 PF01619: Pro_dh" amino acids 196 to 489 (294 residues), 337.6 bits, see alignment E=1.4e-104 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 525 to 1030 (506 residues), 698.8 bits, see alignment E=1.8e-214 PF00171: Aldedh" amino acids 574 to 1029 (456 residues), 379 bits, see alignment E=5.7e-117

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 97% identity to kko:Kkor_2154)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1052 amino acids)

>B158DRAFT_0840 L-proline dehydrogenase (EC 1.5.99.8)/delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) (Kangiella aquimarina DSM 16071)
MFKASQILSDSYQQLSLSEWQEIITQNYSVDESGYLQELLELATPDDKTVNIITHQASQL
VEEIRQSSKSREGVEAFLQQYSLSTKEGVILMCLAEALLRIPDANVANALIRDKLSAAEW
KKHTGQSESILVNASTWGLMLTGKIVDVDQDGDGKPDTLLKGLIAKFGEPVIRQAMNQAM
RLMGRQFVLGRSIKEAMKRGAKSVEKGYTHSFDMLGEAAYTAQDAHRYYEAYSKAISEIG
QVKVKEGQLAPSISIKLSALHPRYEVGQKDRVMTEMVDTLLKLVKQARELDVAVTIDAEE
ADRLELSLDIFEALYTSEICQDWAGLGMVVQAYSKRALPVLGWLDALSRKHGRRIPIRLV
KGAYWDSEIKWSQQAGLSNYPVFTRKAGTDVSYLACARFIMSTKDSFYPQFATHNAQTVF
SIMEMAGDRRDFEFQRLHGMGEILYDKVLERYPGLKCRIYAPVGNHKDLLPYLVRRLLEN
GANTSFVHQLVDKATPVMSLVEHPCLVLKRYPTLHNDRIPLPPHIYGPDRVNSAGTNLHI
NSQIEPFMQNVQQYLDNQWHAKPIIDGKEITTNEEAVFCPYDNTHQIGTVHKVSEELALE
ALTIAHKNFIAWDMTPVEQRAQILEKIADVFEENEAELIALCSRDGGKTMQDGIDEVREA
VDFCRYYANNARKDFGQEITLPGPTGESNHLYLQGRGVFACISPWNFPLAIFTGQVVAAL
VAGNTVLAKPADQTTLVAYRAVQLMHQAGVPTSVLQFVPCRGSTFGKVVLSDIRIAGVAF
TGSTSTAQTINRTLATRDGMLSPLIAETGGQNAMIVDSSALPEQVVADVIQSAFTSAGQR
CSALRVLYVQEDVADRILEVLSGAMQELKLGDPTDLSTDVGPVIDAVAREELAGHVEQLR
ATGNLIAETPMPAGLEKGCFLAPTAFFINHINDLTQEWFGPILHVIKYKSKELDQVIDQI
NGYGFGLTLGIHSRNESTATYIDKRARVGNVYINRNMIGATVGVQPFGGQGLSGTGPKAG
GPFYLHRFATEHTRTNNTAAIGGNATLLSLGD