Protein Info for B158DRAFT_0834 in Kangiella aquimarina DSM 16071

Annotation: tRNA nucleotidyltransferase/poly(A) polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 PF01743: PolyA_pol" amino acids 3 to 122 (120 residues), 73.2 bits, see alignment E=3.8e-24 PF12627: PolyA_pol_RNAbd" amino acids 149 to 211 (63 residues), 46.9 bits, see alignment E=2.8e-16 PF01966: HD" amino acids 229 to 310 (82 residues), 36.8 bits, see alignment E=6.6e-13

Best Hits

Swiss-Prot: 58% identical to CCA_YERE8: Multifunctional CCA protein (cca) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 92% identity to kko:Kkor_2160)

MetaCyc: 57% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (411 amino acids)

>B158DRAFT_0834 tRNA nucleotidyltransferase/poly(A) polymerase (Kangiella aquimarina DSM 16071)
MQIYLVGGAVRDRLLGLKVVDHDHVVVGATPEQMIAKGFKEVGKDFPVYLHPKTGEEYAL
ARTERKSGKGYKGFNVDFSPDITLEQDLIRRDLTINAMAQDKDGNLIDPFNGQQDLRDKI
LRHVSPAFAEDPLRVLRVARFAARFTHLGFIIADETFALMQELSYGDELKHLTSERVWAE
TEKALATQSPQVYFDVLHQCGALAVLMPEIDCLWGIPNPEKWHPEIDTGIHTLMVIEQAA
KKTPDLRIRFAAAVHDLGKGVTPREQWPQHRGHEESGIPIIKKLCKRLKVPKVYEQLATQ
VSRYHLHSHKMFELRPSTVLKMLKGLSAYRNPDFLEAFILTCEADFNGRLGNEEKPYPQG
DLLRECFKVSETIDVQSLIEKGYEGAKLGEAIDRARLKAIKGLLAHKYDSE