Protein Info for B158DRAFT_0819 in Kangiella aquimarina DSM 16071

Annotation: DNA primase, catalytic core

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR01391: DNA primase" amino acids 4 to 414 (411 residues), 486.3 bits, see alignment E=3.6e-150 PF01807: zf-CHC2" amino acids 5 to 100 (96 residues), 135.8 bits, see alignment E=1.5e-43 PF08275: DNAG_N" amino acids 122 to 248 (127 residues), 144.4 bits, see alignment E=8e-46 PF13662: Toprim_4" amino acids 256 to 321 (66 residues), 63.2 bits, see alignment E=8.9e-21 PF01751: Toprim" amino acids 257 to 322 (66 residues), 53.6 bits, see alignment E=8.1e-18 PF13362: Toprim_3" amino acids 257 to 352 (96 residues), 29.8 bits, see alignment E=2.7e-10 PF13155: Toprim_2" amino acids 259 to 345 (87 residues), 66.5 bits, see alignment E=8.8e-22 PF10410: DnaB_bind" amino acids 365 to 419 (55 residues), 37.6 bits, see alignment 8.4e-13 PF08278: DnaG_DnaB_bind" amino acids 453 to 577 (125 residues), 72.2 bits, see alignment E=2.2e-23

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 92% identity to kko:Kkor_2175)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (591 amino acids)

>B158DRAFT_0819 DNA primase, catalytic core (Kangiella aquimarina DSM 16071)
MGLIPQHFIHELLARVDIVDLIDSRVPLKKAGANYKACCPFHGEKTPSFTVSQDKQFYHC
FGCGLHGNAIGFLMEYDRLEFPDAVEELAAMMGMEVPREETVSNAPKVDTSLYDIMEKAA
NYYQQQLKSNKSAIEYLKGRGLSGEIAKQFAIGYVPGEWRNLEAIFPKLQQDKKLQQQLV
ECGLMIRKDSSLYDRFRDRIMFPIKDKRGRVIAFGGRVMGQGEPKYLNSPETPIFHKSNE
LYGLYQARQANRKLSRLLIVEGYMDVVALAQFGINYAVATLGTATTSSHLQQLFRTTREL
ILCFDGDRAGRDAALRAIEHGLPQMREGREIRLMFLPDGEDPDSMVRKVGKEQFEEMIGK
ATPLFEFILEHLSSQVDLASVSGRGQLVHLAKPYLDKITDPIYHEFFSSALAEKAKLSES
KLAAIIEAPTQSQPARKPTQSKPQSTKTNKTLQLAITLLLQQPSLALSLKNYDWLLTLPD
KGSKLLHQLLEIIHKTPKINTSLLLENWRDNPKLYDRLASLAMTEREERIQGQEQQELQD
CVNRLLLHARKIRVDYLQQKAAEHGLTNQEKSEYRQLLLLAKQGSNGNSAK