Protein Info for B158DRAFT_0799 in Kangiella aquimarina DSM 16071

Annotation: aconitate hydratase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 20 to 898 (879 residues), 1327.7 bits, E=0
• PF00330: Aconitase
  • amino acids 74 to 567 (494 residues), 531.5 bits, E=2.2e-163
• PF00694: Aconitase_C
  • amino acids 697 to 823 (127 residues), 147.4 bits, E=3e-47

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 93% identity to kko:Kkor_2204)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (19/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle III (animals) (8/10 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (19/26 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• reductive TCA cycle I (6/11 steps found)
• mixed acid fermentation (9/16 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (11/19 steps found)
• ethene biosynthesis V (engineered) (14/25 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (901 amino acids)

>B158DRAFT_0799 aconitate hydratase 1 (Kangiella aquimarina DSM 16071)
MSNLAQDCKQQLKVGGKSFDIWSLAALDQKGHGIKKLPFSIRILLENALRNRDGLGVTDE
HIDTLLNWEPIPKQEEVPYKPARVLMQDFTGVPAIVDLASVRQEASRHGVDAKKINPLIP
VDLVIDHSVQVDYFGSKSSLEQNINMEYERNRERYQFLKWAQTAFNNFTVVPPGMGICHQ
VNLEYLAQGIVERDGALFPDTLVGTDSHTPMVNGIGVVAWGVGGIEAEAAILGQPIYFIM
PEVVGLKLTGNLPLGTTATDLVLTITELLRKHGVVGKFVEVFGDGLDNLTVTDRATISNM
SPEFGCTVTYFPIDDRTLDYMRDTNRDEAVINRVETYCKNNMLWRADEDQIRYSSVVELD
LSSVVPTVSGPKRPQDKIEVTNLKAQFQSLMELNYGRKYQLLEDRTPADNDKGIIKTVDV
PGEDYQLHDGSIAIAAITSCTNTSNPSVMLGAGLVAKKANDMGLKVKPWVKTSLAPGSKV
VTDYLEHSGLLDDLEALNFFLVGYGCTSCIGNSGPLPDAIGKAVKENDMVVSSVLSGNRN
FEARVHPDVKMNFLMSPMLVVMYALAGRVDIDFDSEPVTYTVDDKPVYFKDLWPSNEEIQ
QIMSEVLSPADYAKSYGEIFEGNEQWKNLEVSKDKVYQWDDQSTYIKQAPFFKGLTPEID
VPGNIEGARVLLKLGDSITTDHISPAGGFSESTPAGKYLLDKGVEKRLFNSYGSRRGNDE
VMVRGTFANVRIKNQLVDKEGGYTRYIPNGETMTVFDAAMKYQESNTPLVVLAGKEYGSG
SSRDWAAKGTTLLGIKAVIAESYERIHRSNLVGMGVLPLQFKPGEDAETLGLRGDETFNI
LGLDKGIETGQTVQVEAVANDESGKVIKFEAISRLDSRVELEYYKNGGILHYVLRQFINQ
A