Protein Info for B158DRAFT_0778 in Kangiella aquimarina DSM 16071

Annotation: DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 PF01149: Fapy_DNA_glyco" amino acids 1 to 114 (114 residues), 119.5 bits, see alignment E=1.9e-38 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 268 (268 residues), 315.3 bits, see alignment E=1.8e-98 PF06831: H2TH" amino acids 129 to 219 (91 residues), 101.8 bits, see alignment E=2.5e-33 PF06827: zf-FPG_IleRS" amino acids 242 to 268 (27 residues), 29.6 bits, see alignment (E = 7.5e-11)

Best Hits

Swiss-Prot: 56% identical to FPG_MAGMM: Formamidopyrimidine-DNA glycosylase (mutM) from Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 89% identity to kko:Kkor_2225)

MetaCyc: 58% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (269 amino acids)

>B158DRAFT_0778 DNA-(apurinic or apyrimidinic site) lyase (EC 4.2.99.18) (Kangiella aquimarina DSM 16071)
MPELPEVETTKNGLAPHIVGKPIKAVNIYQPQLRWPIAVEAKALVGITSGEIRRRAKYML
WEFPSGSLVMHLGMSGTMRVVSAQTALKKHDHFEVVFEDGSALRFNDPRRFGAILWQPAG
ETLSVLSQLGPEPLSDDFDGKYLHTALSKRKGAIKNAIMDNKVVVGVGNIYASESLFMSG
IHPKRAANKVSLARCNLLAGFIKAVLEKAIGEGGTTLKDFTQTDGSPGYFAQQLNVYGRA
DLPCNQCGSLIKKQIIGQRSSFYCPKCQR