Protein Info for B158DRAFT_0733 in Kangiella aquimarina DSM 16071

Annotation: The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1052 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 387 (22 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details amino acids 470 to 493 (24 residues), see Phobius details amino acids 543 to 563 (21 residues), see Phobius details amino acids 612 to 634 (23 residues), see Phobius details amino acids 874 to 893 (20 residues), see Phobius details amino acids 899 to 919 (21 residues), see Phobius details amino acids 975 to 996 (22 residues), see Phobius details amino acids 1006 to 1029 (24 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1045 (1045 residues), 1373.2 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1030 (1030 residues), 1162.5 bits, see alignment E=0 PF03176: MMPL" amino acids 273 to 518 (246 residues), 50.9 bits, see alignment E=1.7e-17 PF02355: SecD_SecF" amino acids 873 to 1024 (152 residues), 21.4 bits, see alignment E=2.2e-08

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 76% identity to kko:Kkor_2271)

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1052 amino acids)

>B158DRAFT_0733 The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family (Kangiella aquimarina DSM 16071)
MAQFFINRPIFAWVIALLILLGGTLAIQQLPVMSYPDISPPQISVTASYPGASAQVIEDT
VTTIIEEEMNGIEGLKYIRSESSRTGTGTINLTFDTGTDIDIAGVEVQNRLKRVEARLPS
SVRTQGVAVDKARRDFLMVVSLYSPNGTFNSTDLGDYLDRAILSDIRRIDGVGSAQQFGS
RYAMRVWLDPKQMAAYGITPAEVNAAVSSQNAQLATGELGSLPSPGNQQINATVLVPAQL
STVEEFGDIILRSSSQGAVVRLKDVATIERGANSYGSQAFLSGQDAAAFAIKLSNTGNAL
DTAEAVKAKMEELEQFFPDDMAWVVPFDASIFVDESISEVVKTLLEAVVLVTVVMFLFLQ
SWRATLIPLIVVPISLIGAGIGLQLFGFSINMLTMFAMVLAIGIVVDDAIIVVENVKRVM
DEEGLKPYPATKKAMKQISGAIIGTTAVLIAVFIPMAFFSGSVGRIYQQFSLTIVISVAI
SAFLSLSLSPAIAQGLLKLEKRDAEGNKAKKEMAFFRWFNRGFESFKNFYMNSVQKMFSK
KGMLITMSVFVLLCGYVVARFIYMPTAFVPSEDQGFIVSTTLLPPGATRERTLELTEKTD
AWYLEQPEVVRVITVAGFSFFGSGQNTAITFVNFKPWSEREGMRNADEFAAAASAEFQKF
SEGNTFAFNMPPIPGLGNSSGFDFQLQNRGTGGQDALFAAAFQIIGKAQQTGKFAEVRPD
TLPAAPMLTLEVDRVKARAMGIDIPTLNSTLQIALGSSYVNDYIDNGKVRQVWVQADAQT
RSTTEEIMSLQIRNQQGDLVNLSEIATASWGEAPAKLTRYNGLPSLPLTGSGAEGVSSGV
VMDLMEQFAAELPPGIAYEWSGQSLEEKVAGNQTALLFALSFLVVFLVLAALYESWSVPM
AVVLMVPLGILGCIIGMDIRGMPNDIYFKVGLITIIGLSAKNAILIVEFAREAQSDGKSP
LEAVIEACKIRLRPILMTSLAFIMGVLPMALASGAGSASRQAIGTGVVSGMFAAAVFSIF
FVPVFYLLVRKIFPRKLKHYEMAARGMEINDD