Protein Info for B158DRAFT_0645 in Kangiella aquimarina DSM 16071

Annotation: NADH:ubiquinone oxidoreductase, Na(+)-translocating, D subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 42 to 62 (21 residues), see Phobius details amino acids 73 to 95 (23 residues), see Phobius details amino acids 102 to 120 (19 residues), see Phobius details amino acids 135 to 155 (21 residues), see Phobius details amino acids 178 to 199 (22 residues), see Phobius details TIGR01939: NADH:ubiquinone oxidoreductase, Na(+)-translocating, D subunit" amino acids 5 to 208 (204 residues), 333.3 bits, see alignment E=3.1e-104 PF02508: Rnf-Nqr" amino acids 10 to 196 (187 residues), 201.5 bits, see alignment E=5.4e-64

Best Hits

Swiss-Prot: 74% identical to NQRD_PSEA6: Na(+)-translocating NADH-quinone reductase subunit D (nqrD) from Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087)

KEGG orthology group: K00349, Na+-transporting NADH:ubiquinone oxidoreductase subunit D [EC: 1.6.5.-] (inferred from 99% identity to kko:Kkor_2357)

MetaCyc: 65% identical to Na(+)-translocating NADH-quinone reductase subunit D (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit D (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (211 amino acids)

>B158DRAFT_0645 NADH:ubiquinone oxidoreductase, Na(+)-translocating, D subunit (Kangiella aquimarina DSM 16071)
MAFDKQEAKSVLFSPIFNNNPIALQVLGICSALAVTSKLETALVMSLALTTVVAFSNLLI
SLIRKQIPSSIRIIVQMTIIASLVILVDQVLKAYAYEVSKQLSVFVGLIITNCIVMGRAE
AYAMKNGPAMSFLDGLGNGLGYSVLLIIVGIIRELFGSGKLLDFTIFPTIADGGWYHTNG
LLMLPASSFFIIGLLIWALRTWKTDQVEDAE