Protein Info for B158DRAFT_0630 in Kangiella aquimarina DSM 16071

Annotation: Cyclopropane fatty acid synthase and related methyltransferases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 344 PF02353: CMAS" amino acids 63 to 326 (264 residues), 148.2 bits, see alignment E=1.5e-46 PF05175: MTS" amino acids 98 to 219 (122 residues), 35.6 bits, see alignment E=3.7e-12 PF05401: NodS" amino acids 105 to 204 (100 residues), 28.5 bits, see alignment E=6.5e-10 PF13489: Methyltransf_23" amino acids 105 to 254 (150 residues), 61.6 bits, see alignment E=3.9e-20 PF03848: TehB" amino acids 117 to 197 (81 residues), 25.6 bits, see alignment E=3.7e-09 PF13847: Methyltransf_31" amino acids 118 to 226 (109 residues), 47.8 bits, see alignment E=7e-16 PF02390: Methyltransf_4" amino acids 121 to 177 (57 residues), 29.1 bits, see alignment E=3.4e-10 PF13649: Methyltransf_25" amino acids 122 to 216 (95 residues), 61.1 bits, see alignment E=7.1e-20 PF08241: Methyltransf_11" amino acids 123 to 219 (97 residues), 51.8 bits, see alignment E=5.4e-17 PF08242: Methyltransf_12" amino acids 123 to 217 (95 residues), 53.1 bits, see alignment E=2.2e-17

Best Hits

Swiss-Prot: 36% identical to PNMT_THLFG: Pavine N-methyltransferase from Thalictrum flavum subsp. glaucum

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 91% identity to kko:Kkor_2372)

MetaCyc: 49% identical to phenylalkylamine N-methyltransferase (Ephedra sinica)
2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase-like protein, clusters with FIG005069"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79 or 2.1.1.M76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (344 amino acids)

>B158DRAFT_0630 Cyclopropane fatty acid synthase and related methyltransferases (Kangiella aquimarina DSM 16071)
MIAQAINLIEKGLLPDRLVRLGIRKLCEQRLTEESAYDCEQQAERYHAFLQELKSSEVAI
KTDKANEQHYEVPAQFYHYSLGKNLKYSSAYWPENVNNLDDAEQAMLDLYAKRGEFVDGQ
DILELGCGWGSLTLYLAAKYPNSTITGVSNSNSQREHIQQQAKERGLTNITIITQDINQF
DPGRQFDRIISIEMFEHVRNYEVLFERVSQWLKDEGKVFLHVFCHRYLMYPFLEDGDDNW
MGRYFFSGGQMPAADTFLNFQQKLQLERRWLVNGQHYEKTSNAWLENMDKHQKQIMPLFE
TVYGKDFAKIWFQRWRIFFMACAELFGYADGNEWMVAHYRFAKK