Protein Info for B158DRAFT_0627 in Kangiella aquimarina DSM 16071

Annotation: Cyclopropane fatty acid synthase and related methyltransferases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 PF02353: CMAS" amino acids 154 to 417 (264 residues), 267.6 bits, see alignment E=4.2e-83 PF05175: MTS" amino acids 190 to 327 (138 residues), 23.5 bits, see alignment E=1.3e-08 PF13489: Methyltransf_23" amino acids 208 to 320 (113 residues), 37.2 bits, see alignment E=8.5e-13 PF08242: Methyltransf_12" amino acids 215 to 309 (95 residues), 42.2 bits, see alignment E=4.1e-14 PF08241: Methyltransf_11" amino acids 215 to 312 (98 residues), 43.1 bits, see alignment E=2e-14 PF13649: Methyltransf_25" amino acids 215 to 308 (94 residues), 50.9 bits, see alignment E=7.4e-17

Best Hits

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 87% identity to kko:Kkor_2375)

Predicted SEED Role

"S-adenosyl-L-methionine dependent methyltransferase, similar to cyclopropane-fatty-acyl-phospholipid synthase"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (439 amino acids)

>B158DRAFT_0627 Cyclopropane fatty acid synthase and related methyltransferases (Kangiella aquimarina DSM 16071)
MNDFTEKQAITGKQQEQSESVKLPLSEKKQVSGFEKLIRSQVLKALSGIKGGQIIISDPV
GKQFCGTANAQSDLCVTISISQLDFYRQVAMHGSIGAAESYMKEQWSTDDLTGLVRLLVI
NRDILDEMEGGLAWLKNSILKLWHFFNRNSVEGSRKNISAHYDLGNDFFKLFLDEHLMYS
SAIFTEQANTLELASELKLKTICEKLDLNANDRLVEIGTGWGGFAIYAAKNYGCHVTTTT
ISEQQYQHARQRVKEEGLESKITILKEDYRKLEGQFDKLVSIEMIEAVGHQYLDTYIKQC
SALLKPDGMALIQAITIEDSRYKQALKSVDFIKRYIFPGSFIPCVSVIVDSARKSSDLRL
INLEDFGDSYAKTLNEWRKRFFAHIEAVKAQGFDQSFIQMWDFYLCYCEGGFIEKAISDV
HLLFAKPGNKRQQWLALQS