Protein Info for B158DRAFT_0468 in Kangiella aquimarina DSM 16071

Annotation: glycyl-tRNA synthetase beta chain (EC 6.1.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 685 (685 residues), 846.9 bits, see alignment E=8.7e-259 PF02092: tRNA_synt_2f" amino acids 6 to 550 (545 residues), 713.9 bits, see alignment E=1.6e-218 PF05746: DALR_1" amino acids 580 to 675 (96 residues), 36.1 bits, see alignment E=6.1e-13

Best Hits

Swiss-Prot: 53% identical to SYGB_ACTP2: Glycine--tRNA ligase beta subunit (glyS) from Actinobacillus pleuropneumoniae serotype 5b (strain L20)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 82% identity to kko:Kkor_2539)

MetaCyc: 53% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (686 amino acids)

>B158DRAFT_0468 glycyl-tRNA synthetase beta chain (EC 6.1.1.14) (Kangiella aquimarina DSM 16071)
MSQYDLLFELGTEELPPKALKTLSEALRDNLAANLQSNDFTFDSIENYATPRRLALIIRN
LADSQPDKNVERLGPAVAAAFNDDGSAKPAAVGFAKSCGVEVDQLDRIETDKGERLGYTI
AQKGQPLSELIEEMLNSALKKLPISKPMRWGNSSAQFIRPVHWTVLLHGSSVLEATILDT
KTGNVSRGHRFMSEGSVSLHDADDYVNKLKAEHVIVDFEERQQLIEEQVKAEAKKLGGEA
QITPELLDEVTGLVEWPVALTGKFDDEFLSVPAECLISSMAEHQKYFHVLDKDGKLMPNF
ITVSNIESKRPQSVIEGNEKVIRPRLADAMFFFETDKKVSLESHQAHLKNVVFQKQLGSV
QDKAQRVAELASKIAPMIGADAQQAHRAGLLSKCDLATNMVGEFDNLQGIMGTYYARHDG
ENENVAIAMTEQYLPKFSGDQLAKNPVGQCLAIADRVDSLVGIFGIGQAPSGAKDPFALR
RAAIGLIRTIVENQLNLDLTDLIAQSKASFGDTLSNANVEAELLDFILERFRAWYLDQGV
SSNTISAVMALRPTQPLDFDARIKAVQFFQTLPEADSLSAANKRVKNILAKADIEVPGSI
DSGLLLEAEEQALAKTIADAEAKLADMINYQDKLQHLASLRDDVDAFFDKVMVNADDAAI
KANRLAMLKKLQDLFGSVADISLLQN