Protein Info for B158DRAFT_0365 in Kangiella aquimarina DSM 16071

Annotation: DNA gyrase, B subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 TIGR01059: DNA gyrase, B subunit" amino acids 7 to 808 (802 residues), 1026.8 bits, see alignment E=0 PF02518: HATPase_c" amino acids 34 to 172 (139 residues), 66.3 bits, see alignment E=9.7e-22 PF00204: DNA_gyraseB" amino acids 222 to 393 (172 residues), 209.5 bits, see alignment E=7.9e-66 PF01751: Toprim" amino acids 422 to 534 (113 residues), 69.3 bits, see alignment E=8.1e-23 PF21249: GyrB_hook" amino acids 536 to 564 (29 residues), 55.1 bits, see alignment (E = 1.3e-18) PF18053: GyrB_insert" amino acids 568 to 734 (167 residues), 192.1 bits, see alignment E=2.1e-60 PF00986: DNA_gyraseB_C" amino acids 738 to 797 (60 residues), 108.4 bits, see alignment 4.6e-35

Best Hits

Swiss-Prot: 72% identical to GYRB_PSEAE: DNA gyrase subunit B (gyrB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 74% identity to alt:ambt_00020)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (808 amino acids)

>B158DRAFT_0365 DNA gyrase, B subunit (Kangiella aquimarina DSM 16071)
MSENNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTD
IYVTIHSDNSVSVKDNGRGIPTEIHEEEGVSAAQVILTVLHAGGKFDDNSYKVSGGLHGV
GVSVVNALSEELKLTIRRAGKRYEQTYVHGEPQAPLEVVGDAETTGTEIRFKPSNKTFTN
TEYHYDILAKRLRELSFLNSGVSIRLADERSGKQDHFMYEGGIKAFVEYLNSKKTPIHEK
VFHFTHEREEDGITVEVAMQWNDGFQESIFCFTNNIPQRDGGTHLAGFRAALTRGLNNFM
ENEGYNKKGKVNTTGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKTAVEQAMGEHL
KDFLLENPGEAKSVVNKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPAL
SELYIVEGDSAGGSAKQGRDRKNQAILPLKGKILNVEKARFDKMLSSQEVGTLITALGCG
IGREEFDADKTRYHRIIIMTDADVDGAHIRTLLLTFFYRQVPQLIERGYIYIAQPPLYKV
KKGKQEQYLKDDEALENYLTQLALNNTELYVSKDAPPITGEGLERLVNEYRDVQTTIRRL
GRLYPSNILNSMIYHPTLKTDDLKDESKVKDWMETLSVKLNKDQKSGAVRFIFNIRKDNE
LEHYLPHFTMFHHGVESEYLLSTEFFASSDYKKISLLGEKLETLIEEGAFVKRGEKIQEI
ESFKDALDWLMGEARRGQAIQRYKGLGEMNPGQLWETTMDPESRRMLQVTIEDAVAADQL
FTTLMGDQVEPRREFIESNALSVSNLDV