Protein Info for B158DRAFT_0286 in Kangiella aquimarina DSM 16071

Annotation: 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 398 TIGR01822: glycine C-acetyltransferase" amino acids 7 to 397 (391 residues), 696.8 bits, see alignment E=3.9e-214 PF00155: Aminotran_1_2" amino acids 43 to 386 (344 residues), 249.9 bits, see alignment E=4.5e-78

Best Hits

Swiss-Prot: 71% identical to KBL_SALTY: 2-amino-3-ketobutyrate coenzyme A ligase (kbl) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00639, glycine C-acetyltransferase [EC: 2.3.1.29] (inferred from 97% identity to kko:Kkor_0134)

MetaCyc: 71% identical to 2-amino-3-ketobutyrate CoA ligase (Escherichia coli K-12 substr. MG1655)
Glycine C-acetyltransferase. [EC: 2.3.1.29]

Predicted SEED Role

"2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)" in subsystem Glycine Biosynthesis or Glycine and Serine Utilization (EC 2.3.1.29)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (398 amino acids)

>B158DRAFT_0286 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (Kangiella aquimarina DSM 16071)
MNQEFLKHIQQNIDEVKEAGLYKKERVIDSQQAADIHVTTGESVLNFCANNYLGLANSPE
LIKAGQEALDHYGYGMASVRFICGTQDVHKELEKRISEFLGMEDTILYSSCFDANGGLFE
TLLDPEDAVISDALNHASIIDGVRLCKAKRYRYANNDMADLEKQLQQADADGARYKLIVT
DGVFSMDGIIADLKSVCDLADKYNALVMVDDSHAVGFIGEKGKGSHEFCDVMGRVDIITG
TLGKALGGASGGYTSASKEVVEWLRQRSRPYLFSNTLAPVIAAASLKVLDMLENGDELRK
KLRHNSEYFREKMTALGFELVPGEHPIIPVMLGDAALASKFADMMLKEGIYVIGFSFPVV
PKGQARIRTQMSAAHDIEHIDRAIAAFEKVGKELGVIS