Protein Info for B158DRAFT_0243 in Kangiella aquimarina DSM 16071

Annotation: haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 PF00702: Hydrolase" amino acids 13 to 201 (189 residues), 72.1 bits, see alignment E=1.3e-23 PF13419: HAD_2" amino acids 18 to 203 (186 residues), 45.7 bits, see alignment E=1.2e-15 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 98 to 199 (102 residues), 38.5 bits, see alignment E=7.3e-14 PF13242: Hydrolase_like" amino acids 162 to 203 (42 residues), 23.6 bits, see alignment 5.8e-09

Best Hits

KEGG orthology group: K07025, putative hydrolase of the HAD superfamily (inferred from 87% identity to kko:Kkor_0178)

Predicted SEED Role

"Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase" (EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (221 amino acids)

>B158DRAFT_0243 haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E (Kangiella aquimarina DSM 16071)
MTEKIKEIDWQQVKVISFDLDDTLWDNRGVIEKCEQDLLEFLAKEHPPIGEKYDVAAMQA
ISKKLIQEDRPELDNMTVLRKEMIHQMLEQTGGGAALINPAFAVFYGCRSQIEIPQITHD
LLKKLKTKYSLFATSNGNSNLYALGLMDYFEHHFIAGIHGRAKPSPEMLHKICEIKNIEP
QQLLHIGDSYETDIKSSIAADCQHLEIHVNDIGKLYEWVVS