Protein Info for B158DRAFT_0164 in Kangiella aquimarina DSM 16071

Annotation: tRNA-U20-dihydrouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 3 to 319 (317 residues), 425.3 bits, see alignment E=7.3e-132 PF01207: Dus" amino acids 13 to 318 (306 residues), 371.3 bits, see alignment E=1.9e-115

Best Hits

Swiss-Prot: 66% identical to DUSB_VIBPA: tRNA-dihydrouridine synthase B (dusB) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K05540, tRNA-dihydrouridine synthase B [EC: 1.-.-.-] (inferred from 95% identity to kko:Kkor_0250)

MetaCyc: 65% identical to tRNA-dihydrouridine synthase B (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (326 amino acids)

>B158DRAFT_0164 tRNA-U20-dihydrouridine synthase (Kangiella aquimarina DSM 16071)
MRIGPYQLENPLILAPMAGVTDRPFRQLCKELGAGLTVSEMIAANPKLRNTRKSIQRSNH
QGEPGIRSVQIAGAEPEMLAQSAQYNVQNGAEIIDINMGCPAKKVCKKAAGSALLQDPKL
VEDILNAVVSAVEVPVTLKIRTGWNHDNRNGVQIAQIAEQAGIQALAVHGRTRADKYLGN
AEYDTIAAIKQSVSIPVVANGDITSPEKARFVLDYTGVDGLMIGRGAQGNPWIFREVWHY
LQTGEKLSPPSWQEVRQVLLGHINALHEFYGAVMGPRIARKHVSWYLQEQADANEFRRTF
NAIEDGAEQLDVLDRYFEQKQQAIAA