Protein Info for B158DRAFT_0021 in Kangiella aquimarina DSM 16071

Annotation: aspartate carbamoyltransferase (EC 2.1.3.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02729: OTCace_N
  • amino acids 23 to 163 (141 residues), 152.7 bits, E=7.9e-49
• TIGR00670: aspartate carbamoyltransferase
  • amino acids 23 to 333 (311 residues), 305.5 bits, E=1.8e-95
• PF00185: OTCace
  • amino acids 176 to 331 (156 residues), 95.5 bits, E=3.7e-31

Best Hits

Swiss-Prot: 63% identical to PYRB_CELJU: Aspartate carbamoyltransferase (pyrB) from Cellvibrio japonicus (strain Ueda107)

KEGG orthology group: K00609, aspartate carbamoyltransferase catalytic subunit [EC: 2.1.3.2] (inferred from 96% identity to kko:Kkor_0393)

Predicted SEED Role

"Aspartate carbamoyltransferase (EC 2.1.3.2)" in subsystem De Novo Pyrimidine Synthesis (EC 2.1.3.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.3.2

Use Curated BLAST to search for 2.1.3.2

Protein Sequence (353 amino acids)

>B158DRAFT_0021 aspartate carbamoyltransferase (EC 2.1.3.2) (Kangiella aquimarina DSM 16071)
MLELRQLKSHNKHLGARMVFQGQHILSIDQLDREAVERILQVADLMHPYVAREKSCRILD
GAVLANMFFEPSTRTRISFGTAFNLLGGHVRETVGMSTSSLAKGESLYDTAQVLSTYADV
VAMRHPQAGSVSEFAAGSKIPVINGGDGPNEHPTQALLDIYTIFNELNRIQKGTENLRIA
MMGDLKNGRTVHSLTQLLCVFPNVDFTFVAPNALAMPEKVLAVCDKHNHRVTITESLEDG
LQGQDVIYQTRIQEERFETQREADLYRGKYRLNQSAYEQFCDPNTIIMHPLPRDSRPEAN
ELSDDMNDNPNLAIFRQAYFGVKVRMALFALVLGVEDKLSQFEAPVTWHSNKL