Protein Info for Atu6170 in Agrobacterium fabrum C58

Annotation: component of type IV secretion system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 789 TIGR00929: type IV secretion/conjugal transfer ATPase, VirB4 family" amino acids 8 to 783 (776 residues), 870.4 bits, see alignment E=6.2e-266 PF03135: CagE_TrbE_VirB" amino acids 173 to 375 (203 residues), 238.7 bits, see alignment E=3.1e-75

Best Hits

Swiss-Prot: 100% identical to VIRB4_AGRFC: Protein virB4 (virB4) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03199, type IV secretion system protein VirB4 (inferred from 100% identity to atu:Atu6170)

Predicted SEED Role

"ATPase required for both assembly of type IV secretion complex and secretion of T-DNA complex, VirB4"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P17794 at UniProt or InterPro

Protein Sequence (789 amino acids)

>Atu6170 component of type IV secretion system (Agrobacterium fabrum C58)
MLGASGTTERSGEVYLPYVGHVSDHIVLLEDGSIMTMAHVSGMAFELEDAEMRNARCRAF
NTLLRNIADDHVSIYAHLVRHDDVPPSPARHFRSAFSASLSEAFEERVLSGKLLRNDHFL
TLIVSPRAALGKVRRRFTKRYRQKENDLTAQTRNLEDLWHLVAGALEAYGLRRLGIREKQ
DVLFTEVGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGKRGLEIRTPKDSYVGSIYS
FREYPATTRPGMLNVLLSLDFPLVLTQSFSFLTRSQAHSKLSLKSSQMLSSGDKAVTQIS
KLSEAEDALASNEFVLGAHHVSLCIYANDLNNLADRGARARTRLADAGAVVVQEGIGMEA
AYWSQLPGNYKWRTRPGAITSRNFAGLVSFENFPEGSGSGHWGNAIARFRTNGGTPFDYI
PHEHDVGMTAIFGPIGRGKTTLMTFILAMLEQSMVDRAGAVVLFDKDRGSELLVRATGGT
YLALRRGAPSGLAPLRGLENTAASHDFLREWIVALIESDGRGGISPEENRRLVRGIHRQL
SFDPHMRSIAGLREFLLHGPAEGAGARLQRWCRGNALGWAFDGELDEVKLDPSITGFDMT
HLLEYEEVCAAAAAYLLHRIGAMVDGRRFVMSCDEFRAYLLNPKFAAVVDKFLLTVRKNN
GMLILATQQPEHVLESQLGASLVAQCMTKIFYPSPTADRSAYIDGLKCTEKEFQAIREDM
AVGSRKFLLKRESGSVVCEFDLREMREYVAVLSGRANTVRFADQLRKVQGDNPSAWLSEF
MARYHEAKD