Protein Info for Atu6010 in Agrobacterium fabrum C58
Annotation: indole acetamide hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to HYIN2_AGRVI: Indoleacetamide hydrolase (TA-iaaH) from Agrobacterium vitis
KEGG orthology group: K01463, [EC: 3.5.1.-] (inferred from 100% identity to atu:Atu6010)MetaCyc: 88% identical to indoleacetamide hydrolase (Agrobacterium tumefaciens)
Amidase. [EC: 3.5.1.4]
Predicted SEED Role
"Indoleacetamide hydrolase (EC 3.5.1.-)" in subsystem Aromatic amino acid degradation (EC 3.5.1.-)
MetaCyc Pathways
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- acrylonitrile degradation I (1/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (1/2 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (1/3 steps found)
- superpathway of acrylonitrile degradation (1/3 steps found)
- indole-3-acetate biosynthesis II (3/12 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Caprolactam degradation
- Cyanoamino acid metabolism
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- Lysine biosynthesis
- Nicotinate and nicotinamide metabolism
- Nucleotide sugars metabolism
- Phenylalanine metabolism
- Sphingolipid metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.-, 3.5.1.4
Use Curated BLAST to search for 3.5.1.- or 3.5.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q7D2R4 at UniProt or InterPro
Protein Sequence (467 amino acids)
>Atu6010 indole acetamide hydrolase (Agrobacterium fabrum C58) MVPITSLAQTLERLRRKDYSCLELVETLIARCQAAKPLNALLATDWDGLRRSAKKIDRHG NAGLGLCGIPLCFKANIATGIFPTSAATPALINHLPKIPSRVAERLFSAGALPGASGNMH ELSFGITSNNYATGAVRNPWNPSLIPGGSSGGVAAAVASRLMLGGIGTDTGASVRLPAAL CGVVGFRPTLARYPRDRIIPVSPTRDTAGIIAQCVADVIILDQVISGRSAKISPMPLKGL RIGLPTTYFYDDLDADVAFAAETTIRLLANRGVTFVEADIPHLEELNSGASLPIALYEFP HALKKYLDDFVGTVSFSDVIKGIRSPDVANIVSAQIDGHQISNDEYELARQSFRPRLQAT YRNYFRLYQLDAILFPTAPLAAKAIGQESSVIHNGSMMNTFKIYVRNVDPSSNAGLPGLS LPACLTPDRLPVGMEIDGLAGSDHRLLAIGAALEKAINFPSFPDAFN