Protein Info for Atu5500 in Agrobacterium fabrum C58

Annotation: dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01315: Ald_Xan_dh_C
  • amino acids 47 to 160 (114 residues), 75.2 bits, E=7.4e-25
• PF02738: MoCoBD_1
  • amino acids 186 to 408 (223 residues), 173.6 bits, E=6e-55
• PF20256: MoCoBD_2
  • amino acids 433 to 707 (275 residues), 188.3 bits, E=3.4e-59

Best Hits

KEGG orthology group: K11177, xanthine dehydrogenase YagR molybdenum-binding subunit [EC: 1.17.1.4] (inferred from 100% identity to atu:Atu5500)

Predicted SEED Role

"Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR @ 4-hydroxybenzoyl-CoA reductase, alpha subunit (EC 1.3.99.20)" (EC 1.3.99.20)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• superpathway of purines degradation in plants (12/18 steps found)
• purine nucleobases degradation II (anaerobic) (14/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4, 1.3.99.20

Use Curated BLAST to search for 1.17.1.4 or 1.3.99.20

Sequence Analysis Tools

See A9CL40 at UniProt or InterPro

Protein Sequence (767 amino acids)

>Atu5500 dehydrogenase (Agrobacterium fabrum C58)
MSTDSQTLPEAGKLGRWQPAVTSDPLLRKHGALGQGISRIEGPMKVQGKTRFAAEFPYDN
ISYAALAFSTVARGRVIDLNVSAAEAAPGVILVMTHKNAPRMKAPSLMMSSPTAAGASNL
PVMQSDEIHWNGQPIALVLAETQEQADHAASLVTAKYELLPAVTSFDDAKKSPRQLDNLL
GQPPFIDIGDAETALANAEVKVDLVYRTPRHNHNAIELHAATVVWKDDELRVHDASQLLD
LTTGQLADIFGLDVSKVHVTSPYVGGGFGGKCFWDHQILACAAARLAGRPVRIMLSREGV
FRIIGGRTVTEQRVALGAKADGTLDALIHTGTAAMTLHNSCPEQFTFPARHLYAAKTFHI
GQDVADMDMLANTFMRAPGESVGTFALECALDELAEKLGLDPIELRRRIEPEKDPTTGKP
FSSRYLIEAYEKGAEQFGWSKRSQTPRQKREGEWLIGMGCATATYPYHRFPGGAVRIKLT
VDGRVTVSTAVHDMGMGTATAQMQHLAARLGLPLDHVTFEYGDSKLPRGVIAGGSTQTAS
IGGAVIAATEVLVEELIKLSGNDSPLAGLSLLEVEARDGGLSHISDNSLFESYQSILRRA
GREELVCEAEAPAPAEMEAFSMHSYGAQFCEVHVSARTGETRVSRFLGSFDAGQILNSKM
ATSQFKGGIIMGIGLALTEETNFDERTGRIVNASLADYHVPVQMDVPAIEILYTNKPDPQ
APMGARGIGEIGITGVGAAVANAVYNATGVRVRDLPITLDKLTGGLD