Protein Info for Atu5435 in Agrobacterium fabrum C58

Annotation: oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01408: GFO_IDH_MocA
  • amino acids 12 to 142 (131 residues), 88.1 bits, E=7.6e-29
• PF02894: GFO_IDH_MocA_C
  • amino acids 154 to 386 (233 residues), 111.4 bits, E=5.8e-36

Best Hits

Swiss-Prot: 61% identical to YFII_BACSU: Uncharacterized oxidoreductase YfiI (yfiI) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to atu:Atu5435)

Predicted SEED Role

"Myo-inositol 2-dehydrogenase (EC 1.1.1.18)" in subsystem Inositol catabolism (EC 1.1.1.18)

MetaCyc Pathways

• myo-inositol degradation I (6/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (6/10 steps found)
• myo-inositol degradation II (1/5 steps found)
• streptomycin biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.18

Use Curated BLAST to search for 1.1.1.18

Sequence Analysis Tools

See A9CL74 at UniProt or InterPro

Protein Sequence (390 amino acids)

>Atu5435 oxidoreductase (Agrobacterium fabrum C58)
MIYGTRQTKRPLRWAMVGGGRGSQIGYIHRSAALRDRYFDLVAGAFDIDAERGRAFGVEL
GLDEARCYADWRTLFAEEAKRPDGIEAVSIATPNNTHYEITKAALESGLHVVCEKPLCFT
VAEAEDLKRIAEERGRIVGVTYGYSGHQVIEHARELVAAGELGEIRLVNLQFAHGFHNEA
VELQNPATRWRVDPRFAGPSYVLGDIGTHPLYISEVILPDLHIKRLLCARQSFIKTRAPL
EDNAMTLMEYDNGAFGTVWSSAINAGSMHGQKVRIVGSKASIEWWDEQPNQLRFEIQGEP
VRILERGMGYLHAKALADDRIGAGHPEGLFEAWANLYDRFAIAMEARNENDGERLANLRY
PGVEAGVEGVRWVENCVRSADLGGVWVDYQ