Protein Info for Atu5178 in Agrobacterium fabrum C58

Annotation: ABC transporter nucleotide binding/ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 648 transmembrane" amino acids 273 to 294 (22 residues), see Phobius details amino acids 522 to 547 (26 residues), see Phobius details amino acids 572 to 598 (27 residues), see Phobius details amino acids 608 to 631 (24 residues), see Phobius details PF00005: ABC_tran" amino acids 25 to 173 (149 residues), 108.8 bits, see alignment E=6.9e-35 PF12704: MacB_PCD" amino acids 272 to 491 (220 residues), 149.3 bits, see alignment E=3.9e-47 PF02687: FtsX" amino acids 527 to 641 (115 residues), 76.3 bits, see alignment E=4.4e-25

Best Hits

Swiss-Prot: 100% identical to MACB_AGRFC: Macrolide export ATP-binding/permease protein MacB (macB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K05685, macrolide transport system ATP-binding/permease protein [EC: 3.6.3.-] (inferred from 100% identity to atu:Atu5178)

Predicted SEED Role

"Macrolide export ATP-binding/permease protein MacB (EC 3.6.3.-)" in subsystem Multidrug Resistance Efflux Pumps (EC 3.6.3.-)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UKE4 at UniProt or InterPro

Protein Sequence (648 amino acids)

>Atu5178 ABC transporter nucleotide binding/ATPase (Agrobacterium fabrum C58)
MTEPLIRVRGVSRAFPAGDEMVRVLKDVDLDIEAGEMMAIIGASGSGKSTLMNILGCLDR
PTTGSYWIEGRETSKMAVDELAALRRERFGFIFQRYHLLGDLSAASNVEVPAIYAGRSRS
DRHKRAISLLTRLGLAERTGNIPSKLSGGQQQRVSIARALMNGGEIILADEPTGALDTHS
GAEVMKILRELHAEGHTIILVTHDKKIAEHADRVVEISDGVIISDERNVSRSTTARPIRE
HGPSAGWRGAIDRMTEALRMAGAAIWAHKMRSLLTMLGIIIGIASVAAISALGAGSQQQI
LSSISSLGTNTIEVRAGKGFGDLEAGKIRTLVPADAEALVNQPYVDSVTPTVTTSVTVKR
AAVAVNASVTGVGADFFRVRGLELAHGQLFDAQDVIAYSQNVVIDASAARDLFPDRVNPV
GQVILLGTMPARVVGVTKRENSFGPAVDTLTVYAPYTTVMGRMLGRPNVDGITVRIRDDV
DPGNVEAAVTRLIERRHGAKDFFLTNSATIRETIETTTQTLTLLISSVAVISLIVGGIGV
MNIMLVSVTERTKEIGVRVAVGARRSDILSQFLIEAVMVCLVGGLMGIMLALGISALFNL
LSPDFKMIFSPGSIIVAFACSTLIGIVFGFLPARNAAKLDPIEALARD