Protein Info for Atu5100 in Agrobacterium fabrum C58

Annotation: DNA polymerase III, alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1085 TIGR00594: DNA polymerase III, alpha subunit" amino acids 4 to 982 (979 residues), 555.3 bits, see alignment E=1.6e-170 PF02811: PHP" amino acids 6 to 122 (117 residues), 46.4 bits, see alignment E=1.4e-15 PF07733: DNA_pol3_alpha" amino acids 272 to 525 (254 residues), 293.8 bits, see alignment E=3.5e-91 PF17657: DNA_pol3_finger" amino acids 528 to 694 (167 residues), 198.3 bits, see alignment E=1.4e-62 PF14579: HHH_6" amino acids 767 to 858 (92 residues), 75.8 bits, see alignment E=6.4e-25 PF01336: tRNA_anti-codon" amino acids 950 to 1020 (71 residues), 32 bits, see alignment 2.4e-11

Best Hits

Swiss-Prot: 100% identical to DNE22_AGRFC: Error-prone DNA polymerase 2 (dnaE2-2) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to atu:Atu5100)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UKK2 at UniProt or InterPro

Protein Sequence (1085 amino acids)

>Atu5100 DNA polymerase III, alpha chain (Agrobacterium fabrum C58)
MSYAELQVTTHFSFLRGASSAQELFETAKALGIQAIGVVDRNSLAGIVRALEASRATDIR
LVVGCRLDLTDGMSLLVYPTDRAAYSRLTRLITLGKSRGGKNNCILHWDDVIAYSRGMIG
VLVPDLPDATCAAQLRRIAEAFGDRAYVSLCLRRRQNDQMRLHEISNLAARFKVKTVVTN
DVLFHEPGRRQLQDIVTCIRHNTTIDDVGFERERHADRYLKPPEEMERLFSRYSEALART
LEIVRRCKFSLEELTYQYPEEAIVPGKDAQASLEHYVWECAPDRYPEGLPQDVLKTVRHE
LDLIRTMKYAPYFLTVFSIVRFARSQGILCQGRGSAANSAVCYILGITSIDPSTNDLLFE
RFVSQERDEPPDIDVDFEHERREEVIQWIYKTYTKDKAALCATVTRYRAKGAIRDVGKAL
GLPEDVIKALSSGMWSWSEEVPDRNIRELNLNPDDRRLALTLKLAQQLMGAPRHLGQHPG
GFVLTHDRLDDLVPIEPATMKDRQIIEWDKDDVEALKFMKVDVLALGMLTCMAKAFDLIR
EHKGQQLDLSNIEQEDAATYAMIRKADTLGTFQIESRAQMAMLPRLKPRTFYDLVVQVAI
VRPGPIQGDMVHPYLRRREGKEPVEYPTPELEAVLGKTLGVPLFQESAMRVAMVCAGFTG
GEADQLRKSMATFKFTGGVSRFKDKLVSGMVKNGYSPEFAEKTFSQLEGFGSYGFPESHA
ASFALIAYASNYIKCHYPDVFCAALLNSQPMGFYAPAQIVGDAIKHGVEVRPVCVNRSRW
DCTLERIEGSDRHAVRLGFRQVKGLAVADAARIVAARMNNPFASVDDMWRRSSVPTEALV
QLAEADAFLPSLKLERRDALWAIKALRDEPLPLFAAAAEREATAIAEQQEPEVALRQMTD
GHNVIEDYSHTGLTLRQHPVAFLRRDLSARNIIPCAEAMNARDGRWVYTAGLVLVRQKPG
SAKGVMFITIEDETGPANIVVWPSLFEKRRSVVLGSSMMAINGRIQREGEVVHLVAQQLF
DLSADLVGLADRDTGFRLPAGRGDEFAHGGGGPDSRDRQKPVVPRDMFTPDLHIDTLKIK
SRNFQ