Protein Info for Atu4759 in Agrobacterium fabrum C58
Annotation: threonine dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to Y4TJ_SINFN: Putative threonine dehydratase (NGR_a01490) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)
KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 100% identity to atu:Atu4759)MetaCyc: 40% identical to catabolic threonine dehydratase (Escherichia coli K-12 substr. MG1655)
Threonine ammonia-lyase. [EC: 4.3.1.19]; L-serine ammonia-lyase. [EC: 4.3.1.19, 4.3.1.17]
Predicted SEED Role
"Threonine dehydratase, catabolic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Glycine and Serine Utilization or Threonine anaerobic catabolism gene cluster or Threonine degradation (EC 4.3.1.19)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- D-serine degradation (3/3 steps found)
- L-methionine degradation II (3/3 steps found)
- L-serine degradation (3/3 steps found)
- glycine degradation (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- glycine betaine degradation I (6/8 steps found)
- L-cysteine degradation II (2/3 steps found)
- L-tryptophan degradation II (via pyruvate) (2/3 steps found)
- L-threonine degradation V (1/2 steps found)
- glycine betaine degradation III (4/7 steps found)
- L-threonine degradation I (3/6 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- superpathway of L-threonine metabolism (11/18 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- purine nucleobases degradation II (anaerobic) (14/24 steps found)
- hypoglycin biosynthesis (4/14 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
- Cysteine metabolism
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.17, 4.3.1.19
Use Curated BLAST to search for 4.3.1.17 or 4.3.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q7CVS8 at UniProt or InterPro
Protein Sequence (331 amino acids)
>Atu4759 threonine dehydratase (Agrobacterium fabrum C58) MSISADKNQLPVSLAAIERAATRLSGQVERTPLVRSEFLSERCGHPVHLKLETLQPIGAF KLRGAMNAILSLDDAVRRRGLVTASTGNHGRAVAYAAAKLGIPATICMSALVPANKVEAI RMLGAEIRIVGRSQDDAQEEVERLTKNRGLTAIPPFDHADVVAGQGTIGLEVVEDMPELA TILVPLSGGGLAGGIAVAVKALKPRARVIGISMERGAAMHASVKAGRPVSVCEEETLADS LGGGIGLANRVTFALCKTLLDEIVLVSEDEIATGICHASREEDLRVEGAGAVGFAAILAG KIAVSGPAAIIVSGGNIDPAVHKTIIDGGVA