Protein Info for Atu4666 in Agrobacterium fabrum C58

Annotation: HlyD family secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF16576: HlyD_D23" amino acids 47 to 297 (251 residues), 38.9 bits, see alignment E=1.2e-13 PF13533: Biotin_lipoyl_2" amino acids 48 to 93 (46 residues), 51.3 bits, see alignment 1.6e-17 PF13437: HlyD_3" amino acids 212 to 317 (106 residues), 53.6 bits, see alignment E=6.8e-18

Best Hits

Swiss-Prot: 56% identical to YHII_ECOLI: Uncharacterized protein YhiI (yhiI) from Escherichia coli (strain K12)

KEGG orthology group: K01993, HlyD family secretion protein (inferred from 100% identity to atu:Atu4666)

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGZ1 at UniProt or InterPro

Protein Sequence (354 amino acids)

>Atu4666 HlyD family secretion protein (Agrobacterium fabrum C58)
MSKKGLLIGLAVVVLAIAAYFAWSKLTAEGLPSGFVASNGRIEAVEIDISTKTAGRLQDI
MVREGDFVKAGQVLAQMDTAQLEAKKRQAEAQLRRAKIAIDTAHSLVAQREAEKTSALAV
VEQRKAQLDSASKTYARSRQLLTGNAVSQQIVDDSEATEQSARATLASAQASLAASDAAI
NAAKAQIIDAEAAVDAAQADIESIETDIVDATLKSPRDGRVQYRIAQPGEVLSAGGRVLN
LVDLGDVYMTFFLPTAEAGRTSMGSDVRLILDAAPQYTIPAKVSFVADVAQFTPKTVETE
EERQKLTFRVRAQIPQELLQKYIQYVKTGLPGMAYVRLDPQAAWPENLERNLVK