Protein Info for Atu4660 in Agrobacterium fabrum C58

Annotation: alpha-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 PF02056: Glyco_hydro_4" amino acids 4 to 185 (182 residues), 160.4 bits, see alignment E=3.8e-51 PF11975: Glyco_hydro_4C" amino acids 196 to 415 (220 residues), 140.8 bits, see alignment E=6.5e-45

Best Hits

Swiss-Prot: 86% identical to AGAL_RHIME: Alpha-galactosidase (melA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K07406, alpha-galactosidase [EC: 3.2.1.22] (inferred from 100% identity to atu:Atu4660)

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.22

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CVI8 at UniProt or InterPro

Protein Sequence (488 amino acids)

>Atu4660 alpha-galactosidase (Agrobacterium fabrum C58)
MSFKIAIIGAGSVGFTKKLFTDLLCVPEFRDIEVALTDISQHNLDMIKAILEKIVEANGF
PVKVTADTDRRRALEGAKYIISCVRVGGLEAYADDIRIPLKYGIDQCVGDTICAGGILYG
QRNIPVILDFCKDIREVAAPGAKFLNYANPMAMNTWAAIEYGKVDTVGLCHGVQHGAEQI
AEILGAESPSELDYICSGINHQTWFIDLRLNGRRIEKDELVAAFEAHPVFSQQEKLRIDV
LKRFGVYSTESNGHLSEYLPWYRKRPEEIARWIDMSDWIHGETGGYLRHSTETRNWFETE
FPQFLASAAKPIEPAKRSNEHASHILEALETGRVYRGHFNVKNNGVITNLPSDAIIESPG
FVDRFGINMVSGITLPEACAATCMASINVQRMSVHAAVSGDIDLLKLAVLHDPLVGAVAT
PEEVWQMVDEMVVAQARWLPQYADAVPAAKERLAKSTVKTRDWAGAARRNVRSIEELRAE
KSALKKAV