Protein Info for Atu4645 in Agrobacterium fabrum C58

Annotation: vanillin: NAD oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 16 to 472 (457 residues), 480.2 bits, E=5.8e-148
• PF05893: LuxC
  • amino acids 136 to 297 (162 residues), 26.7 bits, E=2.6e-10

Best Hits

Swiss-Prot: 62% identical to VDH_PSEFL: Vanillin dehydrogenase (vdh) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 100% identity to atu:Atu4645)

MetaCyc: 62% identical to vanillin dehydrogenase (Pseudomonas sp. HR199)
Vanillin dehydrogenase. [EC: 1.2.1.67]

Predicted SEED Role

"Salicylaldehyde dehydrogenase (EC 1.2.1.65)" in subsystem Naphtalene and antracene degradation (EC 1.2.1.65)

MetaCyc Pathways

• vanillin and vanillate degradation I (1/2 steps found)
• vanillin and vanillate degradation II (1/2 steps found)
• 4-coumarate degradation (aerobic) (3/5 steps found)
• superpathway of vanillin and vanillate degradation (5/10 steps found)
• 4-coumarate degradation (anaerobic) (2/6 steps found)
• carbaryl degradation (1/6 steps found)
• naphthalene degradation (aerobic) (1/6 steps found)
• naphthalene degradation to acetyl-CoA (4/12 steps found)
• pinoresinol degradation (2/10 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (11/42 steps found)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Biosynthesis of phenylpropanoids
• Naphthalene and anthracene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.65 or 1.2.1.67

Sequence Analysis Tools

See A9CGY1 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Atu4645 vanillin: NAD oxidoreductase (Agrobacterium fabrum C58)
MQTVNLFIGGDHCAAANGKRFERNNPITGDAVTSAAAASVEDATNAADAAAAAFPKWSAT
TPGERRRHLLAAAEALRAAGPQIIQAMMEEIGATEAWAGFNVMLASDMLVEAASLTTHIT
GEVIPSNRPGTMAMALRQPAGVVLSMAPWNAPVILGVRSIATPLACGNTVVMKTSELCPR
THALIIEAVASAGLPKGVLNAISNAPDDAAKIVETLIAHPAVRRVNFTGSTRVGRVIAET
AGRYLKPALLELGGKAPFIVLDDADIDAAVAAAAFGAYMNQGQICMSTERIIVLDSIADT
FVEAFAKKAASLTAGDPREGKTPLGSLVSAEAASRIRVLVDDAVSKGARRVAGGGGDGTM
LDAIAVDGVTPAMRLYSEESFGPVVSIIRAGSIDEAVSIANESEFGLSAAVFGRDQARAL
SVAARIESGICHVNGPTVHDEAQLPFGGVKASGYGRFGGTAGIAEFTELRWVTLQDGPLH
YPI