Protein Info for Atu4586 in Agrobacterium fabrum C58

Annotation: choloylglycine hydrolase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF02275: CBAH" amino acids 30 to 317 (288 residues), 162.9 bits, see alignment E=1.5e-51 PF03417: AAT" amino acids 78 to 205 (128 residues), 32.4 bits, see alignment E=7.6e-12

Best Hits

KEGG orthology group: K01442, choloylglycine hydrolase [EC: 3.5.1.24] (inferred from 100% identity to atu:Atu4586)

Predicted SEED Role

"Choloylglycine hydrolase (EC 3.5.1.24)" (EC 3.5.1.24)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGU9 at UniProt or InterPro

Protein Sequence (355 amino acids)

>Atu4586 choloylglycine hydrolase family protein (Agrobacterium fabrum C58)
MRPKTLPFFSACTAAIFSIATLTTSVAEACTRFVYIGENNQVMTARSMDWKTDVGTNLWV
FPRGMERSGEAGPNSVKWTSKYGSVIASGYDVSTTDGMNEAGLAANVLWLVESSYPDYDG
KSPGLSIAAWAQYVLDNFATVEEAVRVLEKNPFIIVTDSVPGEERLATLHLSLSDASGDS
AIVEYIDGKQVIHHGRQYQVMTNSPTFDEQLALNAYWTQIGGTVMLPGTNRASDRFVRAS
FYANAIPKSENPVEAIASVFSVIRNVSVPYGITTPDQPNISSTRWRTVIDHKRKLYFFES
ALTPNVFWIDMTKLDLSKETGAVKKLDLGANQIHIYSGMANESLKDTKPFKFLGL