Protein Info for Atu4510 in Agrobacterium fabrum C58

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 TIGR04379: myo-inosose-2 dehydratase" amino acids 2 to 289 (288 residues), 370 bits, see alignment E=4.9e-115 PF01261: AP_endonuc_2" amino acids 29 to 275 (247 residues), 90 bits, see alignment E=1.1e-29

Best Hits

Swiss-Prot: 46% identical to IOLE_GEOTN: Inosose dehydratase (iolE) from Geobacillus thermodenitrificans (strain NG80-2)

KEGG orthology group: K03335, inosose dehydratase [EC: 4.2.1.44] (inferred from 100% identity to atu:Atu4510)

MetaCyc: 37% identical to scyllo-inosose dehydratase (Bacillus subtilis subtilis 168)
Myo-inosose-2 dehydratase. [EC: 4.2.1.44]

Predicted SEED Role

"Inosose dehydratase (EC 4.2.1.44)" in subsystem Inositol catabolism (EC 4.2.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.44

Use Curated BLAST to search for 4.2.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGR9 at UniProt or InterPro

Protein Sequence (302 amino acids)

>Atu4510 hypothetical protein (Agrobacterium fabrum C58)
MIRYGTNPIAWSNDDDRTLGAHISLEQCLDETAKIGFDGIEKGHKFPTDPEGLKGVLSPR
GLSFVSGWHSLNLLTDDIESEKKAMQPALDLLKAMGSKVIIVCETSNAIHGADDTALVDR
PRLAAEDWAKFGAGVEALAAFAAEQGITLVYHHHMGTVVESEDEIDLLMKHTGPKTHLLL
DTGHCLFGGGDPVRVAQKYMGRVGHIHAKNVRPVIADQVRNERLSFLEGVRRGVFTVPGD
SEGGVNFPPVLKVAAEHGYSGWLVIEAEQDPDVRNPFEYQSLGLKSLKAFARETGLDKAE
AA