Protein Info for Atu4448 in Agrobacterium fabrum C58

Annotation: sorbitol/mannitol ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 transmembrane" amino acids 60 to 84 (25 residues), see Phobius details amino acids 117 to 142 (26 residues), see Phobius details amino acids 152 to 171 (20 residues), see Phobius details amino acids 200 to 225 (26 residues), see Phobius details amino acids 256 to 276 (21 residues), see Phobius details amino acids 309 to 330 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 149 to 336 (188 residues), 38 bits, see alignment E=7.5e-14

Best Hits

KEGG orthology group: K10228, sorbitol/mannitol transport system permease protein (inferred from 100% identity to atu:Atu4448)

MetaCyc: 64% identical to polyol ABC-type transporter permease component MtlF (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, permease component 1" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CUZ6 at UniProt or InterPro

Protein Sequence (338 amino acids)

>Atu4448 sorbitol/mannitol ABC transporter permease (Agrobacterium fabrum C58)
MPGSEPWGRKLRAARRPLSLSNQNWNEPVLPDMPGAYRDARAHGEVSAMATRNTSGLARV
MLAPSVLLLLVWMIVPLAMTLWFSFQNYNLLNPANVSFAGLFNYQYFYTDPAFFQSIWNT
LLIVGGVLVITVVGGIAIALLLDNDIFGQGIVRIMIISPFFVMPPVAALVWKNMIMHPGY
GVLADLSRFFGLQPVDWFAQFPLLSIIIIVAWQWLPFATLILLTALQSLDGEQKEAAEMD
GANFINRFIYLTLPHLARAITVVILIQTIFLLGVYAEILVTTNGGPGYASTNLAFLIYRT
ALLGYDVGGASAGGIIAVVLANIVAIFLMRAVGKNLDR