Protein Info for Atu4408 in Agrobacterium fabrum C58

Annotation: nitrate reductase catalytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 834 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 5 to 40 (36 residues), 18.6 bits, see alignment (E = 1.9e-07) TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 5 to 833 (829 residues), 1579.7 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 44 to 96 (53 residues), 65.5 bits, see alignment 5.1e-22 PF00384: Molybdopterin" amino acids 100 to 573 (474 residues), 238.2 bits, see alignment E=2.2e-74 PF01568: Molydop_binding" amino acids 720 to 827 (108 residues), 82.5 bits, see alignment E=3.5e-27

Best Hits

Swiss-Prot: 100% identical to NAPA_AGRFC: Periplasmic nitrate reductase (napA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 100% identity to atu:Atu4408)

MetaCyc: 66% identical to nitrate reductase large subunit (Aliivibrio fischeri)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8U7P1 at UniProt or InterPro

Protein Sequence (834 amino acids)

>Atu4408 nitrate reductase catalytic subunit (Agrobacterium fabrum C58)
MSSELTRRNLLKAHAAGIAAATAGIALPAAAQPVPGGVSALQIKWSKAPCRFCGTGCGVM
VGVKEGKVVATHGDMQAEVNRGLNCIKGYFLSKIMYGKDRLETPLLRKKNGVYAKDGEFE
PVSWDEAFDVMAQQCKRVLKEKGPTAVGMFGSGQWTIFEGYAATKLMRAGFRSNNLDPNA
RHCMASAAYAFMRTFGMDEPMGCYDDFEHADAFVLWGSNMAEMHPILWTRIADRRLGFDH
VRVAVLSTFTHRSMDLADIPMVFKPGTDLVILNYIANHIIKTGRVNEDFVRNHTKFVRGV
TDIGYGLRPDDPVEVNAANSADPTKTEAIDFETFKEFVSEYTLEKTAAMTGVEAGFLEEL
AELYADPKRKVMSLWTMGFNQHVRGVWANQMVYNIHLLTGKISEPGNSPFSLTGQPSACG
TAREVGTFAHRLPADMTVTNPEHRKHAEEIWRIPHGIIPEKPGYHAVQQDRMLHDGKLNF
YWVQVNNNVQAGPNTKNETYLGYRNPENFIVVSDAYPTITAMSADLILPAAMWVEKEGAY
GNAERRTHVWHQLVEASGEARSDLWQLVEFSKRFTTDEVWPAEILDANPAYRGKTLYEVL
YKDSDVGKFPLSEINADYKNQESSDFGFYLQKGLFEEYAAFGRGHGHDLAPYDAYHEVRG
MRWPVVDGKETLWRYREGYDPYVKPGEGVKFYGNKDGRAVIIAVPYEPPAESPDQEFDTW
LVTGRVLEHWHSGSMTMRVPELYKAFPGARCFMNADDARKRGLNQGAEIRIVSRRGEIRS
RVETRGRNRMPPGVIFVPWFDASQLINKVTLDATDPISKQTDFKKCAVKIEPVA