Protein Info for Atu4333 in Agrobacterium fabrum C58

Annotation: OmpA-like porin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 190 to 200 (11 residues), see Phobius details amino acids 255 to 276 (22 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 74 to 282 (209 residues), 214.6 bits, see alignment E=1.4e-67 PF09850: DotU" amino acids 75 to 276 (202 residues), 205.3 bits, see alignment E=8.3e-65 TIGR03350: type VI secretion system peptidoglycan-associated domain" amino acids 335 to 470 (136 residues), 167.2 bits, see alignment E=1.9e-53 PF00691: OmpA" amino acids 367 to 464 (98 residues), 48.6 bits, see alignment E=8.9e-17

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 100% identity to atu:Atu4333)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CUN4 at UniProt or InterPro

Protein Sequence (501 amino acids)

>Atu4333 OmpA-like porin (Agrobacterium fabrum C58)
MSTDNPSSWQDLPTVVEITEESRRRNQNARNMAAILEDIVETDEQARPARGKVATLPVDK
LLTGFHFGGEEVPTLVQSAAPLLNLAHLLRFSDAEPDPDQLRRACVEAITRYERDLASAR
ISPERARAAHYVVCATVDDVVLSKPWGVRAGWARSGLVSTFHNDVTGGDRVFDILDHFHQ
SPGANKDLLLLIYLCLSLAFEGRTRVSSKGALELSRIRDSLYKTLIGQYGTFERELSPHW
KGVDARHTPLRTMTALWTLLSLMALAFALGYLFFTLSLNNASDVTFERLAHLPPTETPSV
LISNPPPHPPAQVEIPRVVEPPKTVAPRQPSRLEKLMAFLQPEVDKKLVTLADVNGRLRV
RINNSGLFTTGSADVSPQFRDLIQRIGGALAAEKFRAVVIGYTDTVPIKTVEFPSNWHLS
EARAKAVGDILSQFTGPDAILTEGRADSDPIADNKTEEGRQINRRTEIMVLTNPDEKLSD
AGILSPPIENSSGQQPGEAQP