Protein Info for Atu4312 in Agrobacterium fabrum C58

Annotation: sarcosine oxidase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 996 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 3 to 995 (993 residues), 1637 bits, see alignment E=0 PF13510: Fer2_4" amino acids 16 to 101 (86 residues), 83 bits, see alignment E=6.5e-27 PF12831: FAD_oxidored" amino acids 171 to 221 (51 residues), 43.1 bits, see alignment 1.8e-14 PF01134: GIDA" amino acids 171 to 232 (62 residues), 21.7 bits, see alignment 4.8e-08 PF00890: FAD_binding_2" amino acids 171 to 208 (38 residues), 25.3 bits, see alignment (E = 4.4e-09) PF07992: Pyr_redox_2" amino acids 171 to 437 (267 residues), 53.6 bits, see alignment E=1.2e-17 PF13450: NAD_binding_8" amino acids 174 to 213 (40 residues), 28.5 bits, see alignment (E = 7.4e-10) PF17806: SO_alpha_A3" amino acids 515 to 598 (84 residues), 113.2 bits, see alignment E=2.9e-36 PF01571: GCV_T" amino acids 615 to 879 (265 residues), 230.7 bits, see alignment E=8.8e-72 PF08669: GCV_T_C" amino acids 904 to 988 (85 residues), 64.6 bits, see alignment E=3.2e-21

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 100% identity to atu:Atu4312)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CGE9 at UniProt or InterPro

Protein Sequence (996 amino acids)

>Atu4312 sarcosine oxidase alpha subunit (Agrobacterium fabrum C58)
MNSYRVSGRGRVDAARSVSFTFDGKSYRGVKGDTVASALLANGVHLMGRSFKYHRPRGPV
AAGSEEPNALIGTRRGAGQFEPNTRATVQEIWNGLETTSQNKFPSLNFDIGAVNDMAYML
FSAGFYYKTFMWPKSFWNKVYEPFIRAAAGLGVSPTEEDPDTYASRNLHCDVLIVGGGPA
GLAAARAAAVDGLKVVLVDENAEAGGTLLSEPQAKIDGKPAWTWLADELKTLREQGVKVM
TRTTAIAYYHQNMIGLCEKLTDHLETVPKDTPRERLWRVRARQVVLAQGALEKPLVFHGN
DRPGVMLAGAAQTYLNRYGVKVGNRPVVVTSHDSAWYAAFDLHGAGARVQAIIDTRAKVR
EELVNEARALGIPVKLSHTVTATSGRLRVKSVRVNSVNGSTVAAGQEMACDAVLMSGGWT
PSLHLFSHTQGKLAWDDERATFLPALTNEDCLIAGAGRGLWGIEAALKDGAERGREVVAA
LGKTANVSSHAVEYDRTGSGVSHTELLSDRDASKAKAFVDYQNDVTAKDLRLAVREGMRS
IEHVKRYTTNGMATDQGKMSNINGLNIAAEALGKPQPQVGLTTFRPPYTPTTFGAFAGYH
RGEHFEVTRKTQIDSWAKEHGAVYEPVGQWRRAWYFPKPGEDMDAAVGRECRAVRQSVGI
FDASTLGKIEVVGPDAVEFMNRMYTNPWTKLAPGRCRYGLLLGDDGFIRDDGVIGRMTED
RFHVTTTTGGAARVLNMMEDYLQTEWPDLNVWLTSTTEQWSTIALNGPNAAKLLAPLVEG
VELTEEAFPHMSCLECTVAGMPARLFRVSFTGEIGFEVNVPAPLGRKLWEILWEAGQQYC
ITPYGTETMHVLRAEKGYIIVGQDTDGTVTPYDAAMDWAVGKNKPDFVGKRGLARPDLVA
KGRRHLVGLLTEDRSKLEEGAQIVFDAKQPIPMKMVGHVTSSYHSDAAGQPIALALVEGG
HERMGETVYIPMLDRTIAAKITGMVFVDPENTRLKI