Protein Info for Atu4273 in Agrobacterium fabrum C58

Annotation: acetyl-CoA carboxylase, biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 16 to 123 (108 residues), 132.9 bits, E=1.7e-42
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 16 to 459 (444 residues), 627.5 bits, E=6.4e-193
• PF02786: CPSase_L_D2
  • amino acids 129 to 335 (207 residues), 241 bits, E=2.5e-75
• PF02222: ATP-grasp
  • amino acids 154 to 306 (153 residues), 35.5 bits, E=2.1e-12
• PF02785: Biotin_carb_C
  • amino acids 350 to 455 (106 residues), 131.7 bits, E=2.9e-42

Best Hits

Swiss-Prot: 59% identical to ACCC_HAEIN: Biotin carboxylase (accC) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to atu:Atu4273)

MetaCyc: 58% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• jadomycin biosynthesis (3/9 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• superpathway of mycolate biosynthesis (22/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A9CGC7 at UniProt or InterPro

Protein Sequence (464 amino acids)

>Atu4273 acetyl-CoA carboxylase, biotin carboxylase (Agrobacterium fabrum C58)
MMVEVFKDTTPAERRFDTVLIANRGEIAARIQRACHELGLKTVAICSEADRGASYVQTAD
SFLCIGPSAAGKSYLNKDAILLAARLTSSGAIHPGYGFLSENTAFSDAVEKAGLVFIGPG
ASSIATMGDKIAAKRAMIAAAVPCVPGPDTALPDDPAAVESIAQEIGYPVIVKAAGGGGG
RGMRVVPDAGSLHEAIPTTREEARQAFGSPALYMEKFLQHPRHIEIQVLCDTHGHAVWLG
HRDCSMQRRHQKVVEEAPAPGISPDVIRSVGQACVEACQQIGYRGVGTFEFLYENGAFYF
IEMNTRLQVEHPVTEMTSGIDIVQAQIRAAQGEELVLRQQDVKCEGHAFECRINAEDPQT
FLPSAGVVTGLAWPQGQGIRVDTHIHGGYRVSPYYDSLVAKLIVHAPTRSEAMAKMREAL
AGTSIEGISTNLPFLRALFEDDAFVCGETDIHYLEKWLKQRRVA